Leitão J H, Alvim T, Sá-Correia I
Laboratório de Engenharia Bioquímica, Centro de Engenharia Biológica e Química Lisban, Portugal.
FEMS Immunol Med Microbiol. 1996 Apr;13(4):287-92. doi: 10.1111/j.1574-695X.1996.tb00253.x.
Thirty-one strains of Pseudomonas aeruginosa, isolated from water springs, clinical isolates (some of which were from cystic fibrosis (CF) patients), and two type cultures, were characterized by ribotyping. After restriction of chromosomal DNA of the different isolates with EcoRI and hybridization of Southern transfer blots with 2-acetylaminofluorene labelled Escherichia coli 16S + 23S rRNA probe, eleven different ribopatterns were obtained, representing variations of a dominant profile. This largely predominant pattern included both type cultures, all six isolates from water springs, 33% of the nine CF isolates and 43% of fourteen other clinical isolates most of them from nosocomial infections. When the genomic macrorestriction fingerprints of three mucoid CF isolates, with Asel, Drai or BfrI were compared with those of their spontaneous variants, concerning mucoidy, no differences were detected.
从泉水、临床分离株（其中一些来自囊性纤维化（CF）患者）以及两株标准菌株中分离出31株铜绿假单胞菌，采用核糖体分型法对其进行特征分析。用EcoRI对不同分离株的染色体DNA进行酶切，并用2-乙酰氨基芴标记的大肠杆菌16S + 23S rRNA探针与Southern转移印迹杂交后，获得了11种不同的核糖体图谱，代表了一种优势图谱的变体。这种在很大程度上占主导地位的图谱包括两株标准菌株、来自泉水的所有6株分离株、9株CF分离株中的33%以及14株其他临床分离株中的43%，其中大多数来自医院感染。当比较三株黏液型CF分离株用Asel、Drai或BfrI酶切后的基因组酶切图谱与其黏液型自发变体的图谱时，未检测到差异。
