Zajc I, Sampson J
National Institute of Biology, Ljubljana, Slovenia.
Pflugers Arch. 1996;431(6 Suppl 2):R201-2. doi: 10.1007/BF02346338.
Microsatellite sequences, like minisatellites, are a class of polymorphic DNA fragments that are randomly distributed throughout mammalian genome. Although they express significantly lower variation than minisatellites, they have potential to be used in paternity disputes. However, the inherently lower variability together with the more genetically homogeneous nature of pedigree dogs due to the major inbreeding, raised doubts about the effectiveness of microsatellites in canine paternity (paternage) testing. Using about 20 polymorphic canine specific microsatellites on various dog litters demonstrated, that canine microsatellites provide a perfectly adequate basis for assigning paternity in pedigree breeds. Microsatellite paternity tests are more straightforward to perform and interpret than those based on minisatellites (DNA fingerprinting) and require as little as 0.1 ml of blood. The case presented here was a paternity dispute in labradors, where a mixed paternity was suspected.
微卫星序列与小卫星一样，是一类多态性DNA片段，随机分布于整个哺乳动物基因组中。尽管它们的变异程度明显低于小卫星，但仍有用于亲子鉴定的潜力。然而，由于主要的近亲繁殖，纯种犬固有地较低的变异性以及基因上更同质化的特性，引发了对微卫星在犬类亲子鉴定中有效性的质疑。在各种犬类幼崽中使用约20个多态性犬特异性微卫星证明，犬类微卫星为纯种犬种的亲子鉴定提供了完全足够的基础。微卫星亲子鉴定测试比基于小卫星（DNA指纹识别）的测试更易于执行和解释，并且只需要0.1毫升血液即可。这里呈现的案例是拉布拉多犬的一起亲子鉴定纠纷，怀疑存在混合父系情况。
