犬科动物基因组微卫星的分离与特性分析

Isolation and characterization of microsatellites from the canine genome.

作者信息

Holmes N G, Mellersh C S, Humphreys S J, Binns M M, Holliman A, Curtis R, Sampson J

机构信息

Animal Health Trust, Suffolk, UK.

出版信息

Anim Genet. 1993 Aug;24(4):289-92. doi: 10.1111/j.1365-2052.1993.tb00313.x.

DOI:10.1111/j.1365-2052.1993.tb00313.x

PMID:8239072

Abstract

Microsatellite sequences comprising (dC-dA)n.(dG-dT)n repeats have been isolated from canine libraries and sequenced. Oligonucleotide primers have been synthesized to the microsatellite flanking sequences and used in the polymerase chain reaction to amplify those loci from genomic DNA. The degree of polymorphism of each microsatellite was estimated in a set of unrelated dogs. It is concluded that of the 10 loci studied, nine are sufficiently polymorphic to be useful in genetic studies.

摘要

已从犬类文库中分离出包含(dC-dA)n.(dG-dT)n重复序列的微卫星序列并进行了测序。已针对微卫星侧翼序列合成了寡核苷酸引物，并用于聚合酶链反应以从基因组DNA中扩增这些位点。在一组无亲缘关系的犬中估计了每个微卫星的多态性程度。得出的结论是，在所研究的10个位点中，有9个具有足够的多态性，可用于遗传研究。