Segretain D
Laboratoire d'Histologie-Embryologie, UFR Paris Ouest, Faculté de Médecine, Paris, France.
Acta Anat (Basel). 1995;154(3):224-31. doi: 10.1159/000147772.
The three-dimensional structure of the sarcoplasmic reticulum at the neuromuscular junctions of the rat diaphragm was investigated by means of a selective staining technique allowing studies of thick sections combined with stereoscopic observations. The sarcoplasmic reticulum (SR) has different organizations related to the various regions of the postsynaptic cytoplasm. At the base of the membranous folds or deep region, flattened cisternae are smaller and connected by numerous anastomosed tubules. In the mitochondria-rich region, the SR is composed of large flattened cisternae. Between the postsynaptic folds, the SR is formed of poorly anastomosed thin tubes which give rise to single tubules running close to the plasmalemma. The SR is also associated with the Golgi apparatus present in the deeper mitochondria-rich region. This specific organization of the SR in the postsynaptic cytoplasm of the neuromuscular junctions might, like the Golgi apparatus, be involved in the synthesis of some specific proteins, enzymes and receptors.
采用一种选择性染色技术，结合立体观察，对大鼠膈肌神经肌肉接头处肌浆网的三维结构进行了研究，该技术可用于厚切片研究。肌浆网（SR）在突触后细胞质的不同区域具有不同的组织结构。在膜褶底部或深部区域，扁平池较小，由许多相互吻合的小管相连。在富含线粒体的区域，SR由大的扁平池组成。在突触后褶之间，SR由吻合不良的细管形成，这些细管产生靠近质膜的单个小管。SR还与存在于富含线粒体的深部区域的高尔基体相关。神经肌肉接头突触后细胞质中SR的这种特定组织可能像高尔基体一样，参与某些特定蛋白质、酶和受体的合成。
