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维生素E可延长培养的正常人二倍体细胞的寿命。

Extension of the lifespan of cultured normal human diploid cells by vitamin E.

作者信息

Packer L, Smith J R

出版信息

Proc Natl Acad Sci U S A. 1974 Dec;71(12):4763-7. doi: 10.1073/pnas.71.12.4763.

DOI:10.1073/pnas.71.12.4763
PMID:4531015
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC433977/
Abstract

Inclusion of vitamin E (DL-alpha-tocopherol) in the culture medium for human diploid cells greatly prolongs their in vitro lifespan. The addition of 100 mug of DL-alpha-tocopherol per ml of medium has allowed us to culture WI-38 cells for more than 100 population doublings to date. (These cells normally have an in vitro lifespan of 50 +/- 10 population doublings.) Cells at the 100th population doubling have a normal diploid karyotype, appear to behave in all other respects like young WI-38 cells, and are still actively dividing. We interpret this result as support for the free radical theory of aging.

摘要

在人二倍体细胞的培养基中添加维生素E（DL-α-生育酚）可显著延长其体外寿命。每毫升培养基中添加100微克DL-α-生育酚，使我们至今已将WI-38细胞培养至超过100次群体倍增。（这些细胞正常的体外寿命为50±10次群体倍增。）处于第100次群体倍增的细胞具有正常的二倍体核型，在所有其他方面的表现都与年轻的WI-38细胞相似，并且仍在积极分裂。我们将这一结果解释为对衰老自由基理论的支持。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7274/433977/4bbd4bc9d99d/pnas00075-0128-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7274/433977/4bbd4bc9d99d/pnas00075-0128-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7274/433977/4bbd4bc9d99d/pnas00075-0128-a.jpg

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本文引用的文献

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Age-related accumulation of phosphatidylcholine hydroperoxide in cultured human diploid cells and its prevention by alpha-tocopherol.培养的人二倍体细胞中与年龄相关的氢过氧化磷脂酰胆碱积累及其α-生育酚预防作用
Lipids. 1993 Aug;28(8):775-8. doi: 10.1007/BF02536004.
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