• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Extension of the lifespan of cultured normal human diploid cells by vitamin E: a reevaluation.维生素E对培养的正常人二倍体细胞寿命的延长作用：一项重新评估
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1640-1. doi: 10.1073/pnas.74.4.1640.
2
Extension of the lifespan of cultured normal human diploid cells by vitamin E.维生素E可延长培养的正常人二倍体细胞的寿命。
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4763-7. doi: 10.1073/pnas.71.12.4763.
3
The effect of oxygen and vitamin E on the lifespan of human diploid cells in vitro.氧气和维生素E对体外培养的人二倍体细胞寿命的影响。
J Cell Biol. 1977 Jul;74(1):58-67. doi: 10.1083/jcb.74.1.58.
4
Oxygen-dependent growth declining and effect of vitamin E for human diploid fibroblasts in serum-free, BSA-containing culture.在无血清、含牛血清白蛋白的培养条件下，人二倍体成纤维细胞的氧依赖生长下降及维生素E的作用
Tohoku J Exp Med. 1983 Apr;139(4):389-98. doi: 10.1620/tjem.139.389.
5
Effect of antioxidants on cultured human diploid fibroblasts exposed to cystine-free medium.抗氧化剂对暴露于无胱氨酸培养基中的人二倍体成纤维细胞的影响。
Biochem Biophys Res Commun. 1977 Feb 21;74(4):1582-8. doi: 10.1016/0006-291x(77)90623-4.
6
The influence of culture medium volume on cell density and lifespan of human diploid fibroblasts.培养基体积对人二倍体成纤维细胞密度和寿命的影响。
Exp Cell Res. 1975 Mar 15;91(2):389-92. doi: 10.1016/0014-4827(75)90119-6.
7
[Effect of alpha-tocopherol and its derivatives on the viability of cultured malignant cells].[α-生育酚及其衍生物对培养的恶性细胞活力的影响]
Ukr Biokhim Zh (1978). 1998 Mar-Apr;70(2):37-45.
8
Effects of the incorporation of p-fluorophenylalanine on the in vitro lifespan of human diploid cells.对氟苯丙氨酸掺入对人二倍体细胞体外寿命的影响。
Proc Soc Exp Biol Med. 1979 Sep;161(4):449-51. doi: 10.3181/00379727-161-40571.
9
Effects of tocopherol (vitamin E) acid succinate on morphological alterations and growth inhibition in melanoma cells in culture.生育酚（维生素E）琥珀酸酯对培养的黑色素瘤细胞形态改变和生长抑制的影响。
Cancer Res. 1982 Feb;42(2):550-5.
10
Selenium deficiency in cultured adrenocortical cells: restoration of glutathione peroxidase and resistance to hydroperoxides on addition of selenium.培养的肾上腺皮质细胞中的硒缺乏：添加硒后谷胱甘肽过氧化物酶的恢复及对氢过氧化物的抗性
J Cell Physiol. 1985 Apr;123(1):33-8. doi: 10.1002/jcp.1041230106.

引用本文的文献

1
Effect of Selected Antioxidants on the In Vitro Aging of Human Fibroblasts.抗氧化剂对体外培养的人成纤维细胞衰老的影响。
Int J Mol Sci. 2024 Jan 26;25(3):1529. doi: 10.3390/ijms25031529.
2
Effect of Antioxidants on the Fibroblast Replicative Lifespan .抗氧化剂对成纤维细胞复制寿命的影响。
Oxid Med Cell Longev. 2020 Sep 23;2020:6423783. doi: 10.1155/2020/6423783. eCollection 2020.
3
Approaches towards Longevity: Reprogramming, Senolysis, and Improved Mitotic Competence as Anti-Aging Therapies.抗衰老疗法：重编程、衰老细胞清除和提高有丝分裂能力。
Int J Mol Sci. 2019 Feb 21;20(4):938. doi: 10.3390/ijms20040938.
4
Robust DNA Damage Response and Elevated Reactive Oxygen Species in TINF2-Mutated Dyskeratosis Congenita Cells.TINF2 突变的先天性角化不良细胞中强大的 DNA 损伤反应和升高的活性氧
PLoS One. 2016 Feb 9;11(2):e0148793. doi: 10.1371/journal.pone.0148793. eCollection 2016.
5
Restriction of protein synthesis abolishes senescence features at cellular and organismal levels.蛋白质合成的限制消除了细胞和机体水平上的衰老特征。
Sci Rep. 2016 Jan 5;6:18722. doi: 10.1038/srep18722.
6
Ras and Rac1, frequently mutated in melanomas, are activated by superoxide anion, modulate Dnmt1 level and are causally related to melanocyte malignant transformation.Ras和Rac1在黑色素瘤中经常发生突变，被超氧阴离子激活，调节Dnmt1水平，并与黑素细胞恶性转化有因果关系。
PLoS One. 2013 Dec 16;8(12):e81937. doi: 10.1371/journal.pone.0081937. eCollection 2013.
7
Anomalous antioxidant effects in selenium- and vitamin E-deficient liver mitochondria.硒和维生素 E 缺乏的肝线粒体中的异常抗氧化作用。
Biol Trace Elem Res. 1982 Dec;4(4):303-17. doi: 10.1007/BF02786544.
8
Hypoxia, melanocytes and melanoma - survival and tumor development in the permissive microenvironment of the skin.缺氧、黑素细胞与黑色素瘤——皮肤宽松微环境中的生存与肿瘤发展
Pigment Cell Melanoma Res. 2009 Apr;22(2):166-74. doi: 10.1111/j.1755-148X.2009.00553.x. Epub 2009 Feb 13.
9
Iron accumulation during cellular senescence in human fibroblasts in vitro.体外培养的人成纤维细胞在细胞衰老过程中的铁积累。
Antioxid Redox Signal. 2003 Oct;5(5):507-16. doi: 10.1089/152308603770310158.
10
Age-related accumulation of phosphatidylcholine hydroperoxide in cultured human diploid cells and its prevention by alpha-tocopherol.培养的人二倍体细胞中与年龄相关的氢过氧化磷脂酰胆碱积累及其α-生育酚预防作用
Lipids. 1993 Aug;28(8):775-8. doi: 10.1007/BF02536004.

本文引用的文献

1
SERUM ALBUMIN SUPPLEMENTED MEDIUM FOR LONG TERM CULTIVATION OF MAMMALIAN FIBROBLAST STRAINS.用于哺乳动物成纤维细胞系长期培养的血清白蛋白补充培养基。
Proc Soc Exp Biol Med. 1964 Jul;116:688-92. doi: 10.3181/00379727-116-29346.
2
Extension of the lifespan of cultured normal human diploid cells by vitamin E.维生素E可延长培养的正常人二倍体细胞的寿命。
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4763-7. doi: 10.1073/pnas.71.12.4763.
3
Photosensitization of human diploid cell cultures by intracellular flavins and protection by antioxidants.细胞内黄素对人二倍体细胞培养物的光敏化作用及抗氧化剂的保护作用。
Photochem Photobiol. 1976 Sep;24(3):237-42. doi: 10.1111/j.1751-1097.1976.tb06817.x.

维生素E对培养的正常人二倍体细胞寿命的延长作用：一项重新评估

Extension of the lifespan of cultured normal human diploid cells by vitamin E: a reevaluation.

作者信息

Packer L, Smith J R

出版信息

Proc Natl Acad Sci U S A. 1977 Apr;74(4):1640-1. doi: 10.1073/pnas.74.4.1640.

DOI:10.1073/pnas.74.4.1640
PMID:266202
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC430847/
Abstract

Previously we reported [Packer, L. & Smith, J.R. (1974) Proc. Natl. Acad. Sci. USA 71, 4763-4767] that the lifespan of WI-38 human diploid fibroblasts in vitro was significantly increased by continuously growing the cell cultures in the presence of vitamin E(dl-alpha-tocopherol), but in 19 subsequent subcultivation series we were unable to reproduce these findings. While vitamin E is incorporated into the cells and is able to act effectively as an antioxidant, apparantly is intracellular antioxidant properties alone do not routinely result in an increase of cell lifespan. A synergism between vitamin E and some component(s) in the first of two lots of serum used in the original experiments seems the most likely explanation for our earlier findings.

摘要

此前我们报道过[帕克，L. & 史密斯，J.R.（1974年）《美国国家科学院院刊》71卷，4763 - 4767页]，在维生素E（dl-α-生育酚）存在的情况下持续培养WI-38人二倍体成纤维细胞，其体外寿命显著延长，但在随后的19次传代培养系列中，我们无法重现这些结果。虽然维生素E能进入细胞并有效发挥抗氧化剂的作用，但显然仅其细胞内抗氧化特性通常不会导致细胞寿命延长。原始实验中使用的两批血清中第一批血清里的维生素E与某些成分之间的协同作用似乎是对我们早期发现的最有可能的解释。