维生素E对培养的正常人二倍体细胞寿命的延长作用：一项重新评估
Extension of the lifespan of cultured normal human diploid cells by vitamin E: a reevaluation.
作者信息
Packer L, Smith J R
出版信息
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1640-1. doi: 10.1073/pnas.74.4.1640.
Abstract
Previously we reported [Packer, L. & Smith, J.R. (1974) Proc. Natl. Acad. Sci. USA 71, 4763-4767] that the lifespan of WI-38 human diploid fibroblasts in vitro was significantly increased by continuously growing the cell cultures in the presence of vitamin E(dl-alpha-tocopherol), but in 19 subsequent subcultivation series we were unable to reproduce these findings. While vitamin E is incorporated into the cells and is able to act effectively as an antioxidant, apparantly is intracellular antioxidant properties alone do not routinely result in an increase of cell lifespan. A synergism between vitamin E and some component(s) in the first of two lots of serum used in the original experiments seems the most likely explanation for our earlier findings.
摘要
此前我们报道过[帕克，L. & 史密斯，J.R.（1974年）《美国国家科学院院刊》71卷，4763 - 4767页]，在维生素E（dl-α-生育酚）存在的情况下持续培养WI-38人二倍体成纤维细胞，其体外寿命显著延长，但在随后的19次传代培养系列中，我们无法重现这些结果。虽然维生素E能进入细胞并有效发挥抗氧化剂的作用，但显然仅其细胞内抗氧化特性通常不会导致细胞寿命延长。原始实验中使用的两批血清中第一批血清里的维生素E与某些成分之间的协同作用似乎是对我们早期发现的最有可能的解释。
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