人腮腺唾液蛋白的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳：丹磺酰化、考马斯亮蓝R-250和银染检测方法的比较
Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of human parotid salivary proteins: comparison of dansylation, coomassie blue R-250 and silver detection methods.
作者信息
Beeley J A, Newman F, Wilson P H, Shimmin I C
机构信息
Department of Oral Sciences, University of Glasgow Dental School, UK.
出版信息
Electrophoresis. 1996 Mar;17(3):505-6. doi: 10.1002/elps.1150170314.
PMID:8740168
Abstract
Detection of human parotid salivary proteins by dansylation and UV-transillumination after sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) has been compared with Coomassie Blue R-250 and silver staining procedures. Dansylation gives superior results in terms of both resolution and sensitivity, especially with basic proline-rich proteins (PRPs).
摘要
在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）后，通过丹磺酰化和紫外透照检测人腮腺唾液蛋白，并与考马斯亮蓝R-250和银染方法进行了比较。在分辨率和灵敏度方面，丹磺酰化均能给出更优的结果，尤其是对于富含碱性脯氨酸的蛋白质（PRP）。
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