通过免疫金染色法进行α-胰蛋白酶抑制剂（ITI）表型分析。
ITI (inter-alpha trypsin inhibitor) phenotyping by immunogold staining.
作者信息
Kimura Y, Kido A, Nishizono T, Inoue T, Oya M
机构信息
Department of Legal Medicine, Yamanashi Medical University, Japan.
出版信息
Gene Geogr. 1995 Dec;9(3):191-6.
Polymorphism of Inter-alpha-Trypsin Inhibitor (ITI) was investigated in 685 unrelated Japanese individuals by isoelectric focusing followed by electroblotting and immunogold silver staining. The allele frequency estimates were ITI1 = 0.455 +/- 0.013, ITI2 = 0.516 +/- 0.013, ITI3 = 0.028 +/- 0.0045 and ITI4 = 0.001 +/- 0.001 and the phenotype frequencies fitted the Hardy-Weinberg equilibrium. The ITI system could be regarded as a potential genetic marker in paternity testing and anthropological studies.
采用等电聚焦、电转印及免疫金银染色法，对685名无血缘关系的日本个体进行了α-胰蛋白酶抑制剂（ITI）多态性研究。等位基因频率估计值为：ITI1 = 0.455 ± 0.013，ITI2 = 0.516 ± 0.013，ITI3 = 0.028 ± 0.0045，ITI4 = 0.001 ± 0.001，表型频率符合哈迪-温伯格平衡。ITI系统可被视为亲子鉴定和人类学研究中的潜在遗传标记。
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