Chew B P, Erb R E, Malven P V
J Dairy Sci. 1977 Jun;60(6):981-4. doi: 10.3168/jds.S0022-0302(77)83973-8.
Concentrations of prolactin were similar in aliquots of the same milk sample stored for 2 days at 4 C or --23 C but averaged lower if prepared for assay at 30 C than if prepared at 40 or 50 C. Average deviations in prolactin between duplicate measurements relative to respective treatment averages were lowest generally when technique of mixing included vortexing for 5 s immediately prior to pipetting volumes of milk for assay. The average deviation of differences in prolactin among duplicate measurements relative to average concentrations of prolactin were about the same for colostrum, milk, and blood plasma (11, 17, and 14%) stored frozen. Prolactin can be measured reliably in frozen cow's milk provided samples are warmed to 40 C to 50 C and mixed thoroughly when prepared for assay.
在4℃或-23℃下储存2天的同一份牛奶样本的等分试样中，催乳素浓度相似，但如果在30℃下制备用于检测，则平均浓度低于在40℃或50℃下制备的情况。相对于各自处理平均值，重复测量催乳素时的平均偏差通常在混合技术包括在吸取用于检测的牛奶体积之前立即涡旋5秒时最低。冷冻储存的初乳、牛奶和血浆中，重复测量催乳素的差异相对于催乳素平均浓度的平均偏差大致相同（分别为11%、17%和14%）。如果在制备用于检测的样品时将冷冻的牛奶样品加热到40℃至50℃并充分混合，就可以可靠地测量其中的催乳素。
