Watanabe T
Department of Clinical Nutrition, Faculty of Health Science, Suzuka University of Medical Science and Technology, Japan.
Kansenshogaku Zasshi. 1996 Jun;70(6):574-83. doi: 10.11150/kansenshogakuzasshi1970.70.574.
A single intraperitoneal (ip) inoculation of heat-killed Actinobacillus suis ATCC 15,557 (AS 15,557) into normal and immunosuppressed (dexamethasone-treated) mice led to remarkable nonspecific resistance to ip challenge with lethal doses of opportunistic pathogens such as Pseudomonas aeruginosa, methicillin-resistant Staphylococcus aureus and Candida albicans. The duration of this enhanced protective action and the minimal effective dose, in normal mice, induced by AS 15,557 were superior to those induced by other bacterial immunostimulants such as heat-killed Lavtobacillus casei YIT 9018 (LC 9018) and penicillin-treated Streptococcus pyogenes, Su (OK-432). In immunosuppressed mice; the reduced in vivo killing activity of peritoneal exudate cells (PECs) against P. aeruginosa infection was markedly augmented by ip injection of AS 15,557. The degree of PEC augmentation induced by AS 15,557 was higher than that induced by LC 9018 or by OK-432. The toxicity and histopathological changes associated with AS 15557 were very low, as compared with those by produced by LC 9018 and OK-432. The results suggest that AS 15,557, which showed a strong resistance-enhancing capacity against opportunistic bacterial infections, may be a useful bacterial immunostimulant.
将热灭活的猪放线杆菌ATCC 15557（AS 15557）单次腹腔内（ip）接种到正常和免疫抑制（地塞米松处理）小鼠体内，可使其对铜绿假单胞菌、耐甲氧西林金黄色葡萄球菌和白色念珠菌等致死剂量的机会性病原菌的腹腔内攻击产生显著的非特异性抵抗力。在正常小鼠中，AS 15557诱导的这种增强保护作用的持续时间和最小有效剂量优于其他细菌免疫刺激剂，如热灭活的干酪乳杆菌YIT 9018（LC 9018）和青霉素处理的化脓性链球菌Su（OK-432）。在免疫抑制小鼠中，腹腔注射AS 15557可显著增强腹腔渗出细胞（PEC）对铜绿假单胞菌感染的体内杀伤活性。AS 15557诱导的PEC增强程度高于LC 9018或OK-432诱导的程度。与LC 9018和OK-432产生的毒性和组织病理学变化相比，AS 15557相关的毒性和组织病理学变化非常低。结果表明，AS 15557对机会性细菌感染具有强大的抵抗力增强能力，可能是一种有用的细菌免疫刺激剂。
