Watanabe T, Hotta C
Department of Clinical Nutrition, Faculty of Health Science, Suzuka University of Medical Science and Technology, Mie, Japan.
Hiroshima J Med Sci. 1996 Jun;45(2):63-8.
The carbon clearance ability of female ddY mice was markedly reduced by feeding with a high fat-low carbohydrate (HFLC) diet for 8 weeks. The reduced clearance ability was restored by a single intraperiotoneal (i.p.) injection with heat-killed Lactobacillus casei (YIT 9018) cells (LC 9018). The i.p. injection of LC 9018 into the HFLC diet-fed mice led to a remarkable enhancement of nonspecific resistance when they were exposed to 50% lethal doses of Pseudomonas aeruginosa or herpes simplex virus, type 1. The resistance-enhancing capacity of LC 9018 against P. aeruginosa infection was significantly maintained for 7 days after the ip inoculation with LC 9018 in mice fed the HFLC diet. The reduced in vivo killing activity of peritoneal exudate cells (macrophage-rich) from mice fed the HFLC diet to P. aeruginosa infection was markedly augmented by the i.p. administration of LC 9018. The results indicate that LC 9018, one of the bacterial immunostimulants, may restore the reduced host defense functions in mice induced by feeding with a high fat diet for a long period.
通过高脂低碳水化合物（HFLC）饮食喂养8周，雌性ddY小鼠的碳清除能力显著降低。单次腹腔注射热灭活的干酪乳杆菌（YIT 9018）细胞（LC 9018）可恢复降低的清除能力。给喂食HFLC饮食的小鼠腹腔注射LC 9018，当它们暴露于50%致死剂量的铜绿假单胞菌或1型单纯疱疹病毒时，可显著增强非特异性抵抗力。在喂食HFLC饮食的小鼠中，腹腔接种LC 9018后，LC 9018对铜绿假单胞菌感染的抵抗力增强能力在7天内显著维持。腹腔注射LC 9018可显著增强喂食HFLC饮食小鼠的腹膜渗出细胞（富含巨噬细胞）对铜绿假单胞菌感染的体内杀伤活性。结果表明，作为细菌免疫刺激剂之一的LC 9018可能恢复长期高脂饮食诱导的小鼠中降低的宿主防御功能。
