Sciancalepore M, Constanti A
Biophysics Section, International School for Advanced Studies (SISSA), Trieste, Italy.
Neuroreport. 1995 Dec 15;6(18):2489-94. doi: 10.1097/00001756-199512150-00012.
Whole-cell recordings were made from neurones in slices of rat olfactory cortex (10-19 days old), using potassium methylsulphate-filled pipettes. Positive commands applied from -60 mV in the presence of oxotremorine-M (10-20 microgramM, 20 of 30 cells) or trans-ACPD (10-50 microgramM, 4 of 9 cells) evoked a slow inward tail current similar to the K+ -mediated tail current (I K,ADP) recorded with sharp electrodes. I ADP was reduced by hyperpolarization, showed a 27% decrease in input conductance at its peak and was depressed by Cd(2+), 4AP or high K+ but unaffected by Cs+, Ba(2+) or TEA (5 mM). IADP was significantly larger in cells between postnatal days 13 and 15 than in younger neurones (10-12 days). These data show that stable whole-cell recordings of I ADP can be made from olfactory neurones in cortical slices, and support our hypothesis that IADP is a slowly reactivating, Ca(2+) -sensitive K+ conductance.
使用充满甲硫酸钾的移液管，对10 - 19日龄大鼠嗅皮层切片中的神经元进行全细胞记录。在存在氧化震颤素 - M（10 - 20微克/毫升，30个细胞中的20个）或反式 - ACPD（10 - 50微克/毫升，9个细胞中的4个）的情况下，从 - 60毫伏施加正向指令，诱发了一种缓慢的内向尾电流，类似于用尖锐电极记录的K⁺介导的尾电流（IK,ADP）。IK,ADP通过超极化而降低，在其峰值时输入电导下降27%，并被Cd²⁺、4 - 氨基吡啶或高K⁺抑制，但不受Cs⁺、Ba²⁺或TEA（5毫摩尔）影响。IK,ADP在出生后第13至15天的细胞中比年轻神经元（10 - 12天）中的明显更大。这些数据表明，可以从皮层切片中的嗅觉神经元进行稳定的IK,ADP全细胞记录，并支持我们的假设，即IK,ADP是一种缓慢再激活的、Ca²⁺敏感的K⁺电导。
