Ristow S S, Grabowski L D, Wheeler P A, Prieur D J, Thorgaard G H
Department of Animal Sciences, Washington State University, Pullman 99164-6332, USA.
Dev Comp Immunol. 1995 Nov-Dec;19(6):497-505. doi: 10.1016/0145-305x(95)00029-s.
Nonspecific cytotoxic cell (NCC) activity was assessed in the peripheral blood of four isogenic lines of rainbow trout (Oncorhynchus mykiss) which were derived by the chromosome set manipulation technique of androgenesis. In these fish, whose isogenicity was previously confirmed by multilocus DNA fingerprint analysis, NCC activity was studied by the release of 51Cr from YAC-1 targets. Two groups of trout (the homozygous Arlee 12 line and the heterozygous hybrid of the Arlee 63 and Arlee 12 lines) had significantly lower levels of NCC activity in peripheral blood than either outbred rainbow trout or other lines with Hot Creek or hybrid Arlee x Hot Creek ancestry. The low NCC activity in the Arlee line appears to be inherited as a recessive trait. Peripheral blood cells of the trout mediated lectin dependent cellular cytotoxicity (LDCC) with the addition of phytohemagglutinin to co-cultures of effector cells and YAC-1 cells. The low NCC activity in the peripheral blood of these fish is not due to a condition analogous to the NCC-deficient Chediak-Higashi syndrome of man or the beige mutation of mice.
在通过雄核发育的染色体组操作技术获得的四个虹鳟（Oncorhynchus mykiss）同基因系的外周血中评估了非特异性细胞毒性细胞（NCC）活性。在这些先前通过多位点DNA指纹分析证实了同基因性的鱼中，通过YAC-1靶标释放51Cr来研究NCC活性。两组鳟鱼（纯合的阿利12系以及阿利63系和阿利12系的杂合杂交种）外周血中的NCC活性水平明显低于杂交虹鳟或具有霍特克里克血统或阿利×霍特克里克杂交血统的其他品系。阿利系中较低的NCC活性似乎作为隐性性状遗传。在效应细胞和YAC-1细胞的共培养物中添加植物血凝素后，鳟鱼的外周血细胞介导了凝集素依赖性细胞毒性（LDCC）。这些鱼外周血中较低的NCC活性并非由于类似于人类NCC缺陷的切迪阿克-东综合征或小鼠米色突变的情况所致。
