Henion P D, Raible D W, Beattie C E, Stoesser K L, Weston J A, Eisen J S
Institute of Neuroscience, University of Oregon, Eugene 97403, USA.
Dev Genet. 1996;18(1):11-7. doi: 10.1002/(SICI)1520-6408(1996)18:1<11::AID-DVG2>3.0.CO;2-4.
The neural crest provides a useful model to learn how cell fate diversification is regulated during vertebrate development. Our approach is to isolate zebrafish mutations in which the development of neural crest derivatives is disrupted, in order to learn about the underlying genetic mechanisms. We describe a screen in which parthenogenetic diploid embryos are examined both for visible phenotypes and for cellular defects in neural crest-derived sensory neurons recognized immunohistochemically. We present preliminary results from this screen and briefly describe a few representative mutations. We also discuss the general utility of our strategy and comment on the future directions of this approach.
神经嵴为研究脊椎动物发育过程中细胞命运多样化的调控提供了一个有用的模型。我们的方法是分离斑马鱼突变体，其中神经嵴衍生物的发育受到破坏，以便了解潜在的遗传机制。我们描述了一个筛选过程，其中对孤雌生殖二倍体胚胎进行检查，以观察可见表型以及通过免疫组织化学识别的神经嵴衍生感觉神经元中的细胞缺陷。我们展示了该筛选的初步结果，并简要描述了一些代表性突变。我们还讨论了我们策略的一般实用性，并对该方法的未来方向进行了评论。
