Lin S Y, Jeng Y S, Wang C K, Ko F T, Lin K Y, Wang C S, Liu J D, Chen P H, Chang J G
Department of Internal Medicine, Taipei Municipal Jen-Ai Hospital, Taiwan, ROC.
J Gastroenterol Hepatol. 1996 Mar;11(3):286-9. doi: 10.1111/j.1440-1746.1996.tb00077.x.
Helicobacter pylori has been associated with a variety of upper gastrointestinal diseases. Histopathological examination and culture are considered to be the more specific tests in the diagnosis of H. pylori infection. In the present study, we evaluated the efficiency of a polymerase chain reaction (PCR) assay of the H. pylori urease A gene as a procedure in the diagnosis of gastric H. pylori infection in various gastroduodenal diseases. Biopsy specimens were obtained from the antral mucosa of 83 patients during endoscopic examination and were submitted to three tests for the detection of H. pylori infection. The detection rates of H. pylori using PCR, histopathological examination and culture were 84, 77 and 63%, respectively. When the infection was defined, by the agreement of culture and histopathological examination or by positive culture, the PCR assay had a sensitivity of 98.1% and a specificity of 84.6%. When the infection was defined by a positive result of either two of the three tests or by positive culture, the PCR assay had a sensitivity of 98.6% and a specificity of 85.7%. We conclude that the PCR assay is a valuable test for the diagnosis of H. pylori infection in gastroduodenal diseases.
幽门螺杆菌与多种上消化道疾病相关。组织病理学检查和培养被认为是诊断幽门螺杆菌感染更具特异性的检测方法。在本研究中，我们评估了一种针对幽门螺杆菌尿素酶A基因的聚合酶链反应（PCR）检测方法在诊断各种胃十二指肠疾病中幽门螺杆菌感染的效率。在内镜检查期间从83例患者的胃窦黏膜获取活检标本，并进行三项检测以检测幽门螺杆菌感染。使用PCR、组织病理学检查和培养检测幽门螺杆菌的阳性率分别为84%、77%和63%。当通过培养和组织病理学检查一致或培养阳性来定义感染时，PCR检测的灵敏度为98.1%，特异性为84.6%。当通过三项检测中的两项阳性结果或培养阳性来定义感染时，PCR检测的灵敏度为98.6%，特异性为85.7%。我们得出结论，PCR检测是诊断胃十二指肠疾病中幽门螺杆菌感染的一种有价值的检测方法。
