从具有随机化受体茎序列的RNA文库中选择氨酰化tRNA：让老狗学新把戏。 - Suppr

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超能文献

Selection of aminoacylated tRNAs from RNA libraries having randomized acceptor stem sequences: using old dogs to perform new tricks.

作者信息

Sampson J R, Saks M E

机构信息

Division of Biology, California Institute of Technology, Pasadena 91125, USA.

出版信息

Methods Enzymol. 1996;267:384-410. doi: 10.1016/s0076-6879(96)67024-4.

DOI:10.1016/s0076-6879(96)67024-4

PMID:8743328

Abstract

摘要

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Selection of aminoacylated tRNAs from RNA libraries having randomized acceptor stem sequences: using old dogs to perform new tricks.从具有随机化受体茎序列的RNA文库中选择氨酰化tRNA：让老狗学新把戏。

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Contributions of discrete tRNA(Ser) domains to aminoacylation by E.coli seryl-tRNA synthetase: a kinetic analysis using model RNA substrates.大肠杆菌丝氨酰 - tRNA合成酶对离散tRNA(Ser)结构域氨酰化作用的贡献：使用模型RNA底物的动力学分析

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Interaction of Escherichia coli tRNA(Ser) with its cognate aminoacyl-tRNA synthetase as determined by footprinting with phosphorothioate-containing tRNA transcripts.通过含硫代磷酸酯的tRNA转录本足迹法测定大肠杆菌tRNA(Ser)与其同源氨酰-tRNA合成酶的相互作用。

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Variant minihelix RNAs reveal sequence-specific recognition of the helical tRNA(Ser) acceptor stem by E.coli seryl-tRNA synthetase.变异的小螺旋RNA揭示了大肠杆菌丝氨酰-tRNA合成酶对螺旋状tRNA(Ser)受体茎的序列特异性识别。

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Selenocysteine tRNA and serine tRNA are aminoacylated by the same synthetase, but may manifest different identities with respect to the long extra arm.硒代半胱氨酸转运RNA和丝氨酸转运RNA由同一种合成酶进行氨酰化，但就长的额外臂而言可能表现出不同的特性。

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Rapid selection of aminoacyl-tRNAs based on biotinylation of alpha-NH2 group of charged amino acids.基于带电荷氨基酸α-NH2基团生物素化的氨酰-tRNA快速筛选。

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