从具有随机化受体茎序列的RNA文库中选择氨酰化tRNA：让老狗学新把戏。 - Suppr

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超能文献

Selection of aminoacylated tRNAs from RNA libraries having randomized acceptor stem sequences: using old dogs to perform new tricks.

作者信息

Sampson J R, Saks M E

机构信息

Division of Biology, California Institute of Technology, Pasadena 91125, USA.

出版信息

Methods Enzymol. 1996;267:384-410. doi: 10.1016/s0076-6879(96)67024-4.

DOI:10.1016/s0076-6879(96)67024-4

PMID:8743328

Abstract

摘要

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Selection of aminoacylated tRNAs from RNA libraries having randomized acceptor stem sequences: using old dogs to perform new tricks.从具有随机化受体茎序列的RNA文库中选择氨酰化tRNA：让老狗学新把戏。

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Variant minihelix RNAs reveal sequence-specific recognition of the helical tRNA(Ser) acceptor stem by E.coli seryl-tRNA synthetase.变异的小螺旋RNA揭示了大肠杆菌丝氨酰-tRNA合成酶对螺旋状tRNA(Ser)受体茎的序列特异性识别。

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Rapid selection of aminoacyl-tRNAs based on biotinylation of alpha-NH2 group of charged amino acids.基于带电荷氨基酸α-NH2基团生物素化的氨酰-tRNA快速筛选。

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