Laster S M, Mackenzie J M
Department of Microbiology, North Carolina State University, Raleigh 27511, USA.
Microsc Res Tech. 1996 Jun 15;34(3):272-80. doi: 10.1002/(SICI)1097-0029(19960615)34:3<272::AID-JEMT10>3.0.CO;2-J.
The murine cell line C3HA has been used extensively in studies of the cytopathology that accompanies TNF-induced cytolysis. This cell line undergoes an apoptic form of cell death characterized by plasma membrane blebbing and cytoplasmic boiling. Since plasma membrane blebs also appear on C3HA cells during mitosis, in this report we have compared these blebs with those that appear during apoptosis to determine whether they represent related structures. Our results reveal several differences. During mitosis, the blebs that appear are smaller and more heterogeneous in size than are those that appear during apoptosis. In addition, during mitosis bleb formation is preceded by the appearance of microvilli on the cell surface. No microvilli are observed during apoptosis. The staining pattern with rhodamine phalloidin also differed between mitotic and apoptic blebs, indicating a difference in their content of f-actin. The blebs that form during mitosis stained in a bright, uniform manner, suggesting an association with f-actin. In contrast, apoptic blebs were stained only at their base, the bleb itself being devoid of f-actin-associated staining. This difference may help explain why mitotic blebs are reintegrated into the cell surface, while the blebs that form during apoptosis are not.
小鼠细胞系C3HA已广泛应用于伴随肿瘤坏死因子（TNF）诱导的细胞溶解的细胞病理学研究。该细胞系经历一种凋亡形式的细胞死亡，其特征为质膜起泡和细胞质沸腾。由于在有丝分裂期间C3HA细胞上也会出现质膜泡，在本报告中，我们将这些泡与凋亡期间出现的泡进行了比较，以确定它们是否代表相关结构。我们的结果揭示了几个差异。在有丝分裂期间出现的泡比凋亡期间出现的泡更小且大小更不均一。此外，在有丝分裂期间，泡的形成之前细胞表面会出现微绒毛。在凋亡期间未观察到微绒毛。有丝分裂泡和凋亡泡用罗丹明鬼笔环肽染色的模式也不同，表明它们的丝状肌动蛋白（f-肌动蛋白）含量存在差异。在有丝分裂期间形成的泡以明亮、均匀的方式染色，表明与f-肌动蛋白有关。相比之下，凋亡泡仅在其基部染色，泡本身没有与f-肌动蛋白相关的染色。这种差异可能有助于解释为什么有丝分裂泡会重新整合到细胞表面，而凋亡期间形成的泡则不会。
