Vitzthum H, Homberg U, Agricola H
Institut für Zoologie, Universität Regensburg, Germany.
J Comp Neurol. 1996 Jun 3;369(3):419-37. doi: 10.1002/(SICI)1096-9861(19960603)369:3<419::AID-CNE7>3.0.CO;2-8.
The distribution and morphology of neurons containing allatostatin-related substances in the brain of the locust Schistocerca gregaria was investigated using an antiserum against Diploptera punctata allatostatin I (Dip-allatostatin I, APSGAQRLYGFGL-amide). In each brain hemisphere, about 550 neurons in the midbrain and 500 neurons in the optic lobe exhibit Dip-allatostatin I-like immunoreactivity, including about eight lateral neurosecretory cells with processes to the retrocerebral complex. All major brain areas except the antennal lobe, the mushroom body, and large parts of the lamina, are innervated by Dip-allatostatin I-immunoreactive processes. Immunostaining in the central complex was studied in detail. The central complex is innervated by more than 260 Dip-allatostatin I-immunoreactive neurons belonging to six different cell types, four sets of tangential neurons and two sets of columnar neurons. These neurons give rise to intense immunostaining in the protocerebral bridge, in several layers of the upper division of the central body, and in the dorsalmost layer of the lower division of the central body. Double-label experiments show colocalization of Dip-allatostatin I- and serotonin-like immunoreactivities in one type of columnar and one type of tangential neurons of the central complex. The similar patterns of Dip-allatostatin I- and galanin message-associated peptide-like immunoreactivities result from cross-reactivity of the anti-galanin message-associated peptide antiserum with Dip-allatostatin I. The results provide further insight into the anatomical and neurochemical organization of the locust central complex and suggest a prominent neuroactive role for Dip-allatostatin I-related peptides in this brain area.
使用针对点刻甘薯抑咽侧体素I（Dip-抑咽侧体素I，APSGAQRLYGFGL-酰胺）的抗血清，研究了沙漠蝗（Schistocerca gregaria）脑中含有抑咽侧体素相关物质的神经元的分布和形态。在每个脑半球中，中脑约有550个神经元和视叶约有500个神经元呈现出Dip-抑咽侧体素I样免疫反应性，包括约8个向脑后复合体发出突起的外侧神经分泌细胞。除触角叶、蘑菇体和大部分神经纤维层外，所有主要脑区均有Dip-抑咽侧体素I免疫反应性神经突起支配。对中央复合体中的免疫染色进行了详细研究。中央复合体由属于六种不同细胞类型的260多个Dip-抑咽侧体素I免疫反应性神经元支配，包括四组切向神经元和两组柱状神经元。这些神经元在原脑桥、中央体上部分的几层以及中央体下部分的最背层产生强烈的免疫染色。双标记实验表明，在中央复合体的一种柱状神经元和一种切向神经元中，Dip-抑咽侧体素I和5-羟色胺样免疫反应性共定位。Dip-抑咽侧体素I和甘丙肽信息相关肽样免疫反应性的相似模式是由于抗甘丙肽信息相关肽抗血清与Dip-抑咽侧体素I的交叉反应所致。这些结果为深入了解蝗虫中央复合体的解剖和神经化学组织提供了进一步的线索，并表明Dip-抑咽侧体素I相关肽在该脑区具有重要的神经活性作用。
