Kimmerly W, Stultz K, Lewis S, Lewis K, Lustre V, Romero R, Benke J, Sun D, Shirley G, Martin C, Palazzolo M
Drosophila Genome Center, University of California, Berkeley 94720, USA.
Genome Res. 1996 May;6(5):414-30. doi: 10.1101/gr.6.5.414.
A PCR-based sequence-tagged site (STS) content mapping strategy has been used to generate a physical map with 90% coverage of the 120-Mb euchromatic portion of the Drosophila genome. To facilitate map completion, the bulk of the STS markers was chosen in a nonrandom fashion. To ensure that all contigs were localized in relation to each other and the genome, these contig-building procedures were performed in conjunction with a large-scale in situ hybridization analysis of randomly selected clones from a Drosophila genomic library that had been generated in a P1 cloning vector. To date, the map consists of 649 contigs with an STS localized on average every 50 kb. This is the first whole genome that has been mapped based on a library constructed with large inserts in a vector that is maintained in Escherichia coli as a single-copy plasmid.
一种基于聚合酶链反应（PCR）的序列标签位点（STS）含量作图策略已被用于构建果蝇基因组120 Mb常染色质部分90%覆盖率的物理图谱。为便于完成图谱构建，大部分STS标记是以非随机方式选择的。为确保所有重叠群相互之间以及与基因组的定位关系，这些构建重叠群的程序与对来自以P1克隆载体构建的果蝇基因组文库中随机选择的克隆进行的大规模原位杂交分析相结合进行。到目前为止，该图谱由649个重叠群组成，平均每50 kb定位一个STS。这是第一个基于在大肠杆菌中作为单拷贝质粒保存的载体构建的大插入片段文库绘制的全基因组图谱。
