Ajioka J W, Smoller D A, Jones R W, Carulli J P, Vellek A E, Garza D, Link A J, Duncan I W, Hartl D L
Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110-1095.
Chromosoma. 1991 Sep;100(8):495-509. doi: 10.1007/BF00352200.
We present a strategy for assembling a physical map of the genome of Drosophila melanogaster based on yeast artificial chromosomes (YACs). In this paper we report 500 YACs containing inserts of Drosophila DNA averaging 200 kb that have been assigned positions on the physical map by means of in situ hybridization with salivary gland chromosomes. The cloned DNA fragments have randomly sheared ends (DY clones) or ends generated by partial digestion with either NotI (N clones) or EcoRI (E clones). Relative to the euchromatic portion of the genome, the size distribution and genomic positions of the clones reveal no significant bias in the completeness or randomness of genome coverage. The 500 mapped euchromatic clones contain an aggregate of approximately 100 million base pairs of DNA, which is approximately one genome equivalent of Drosophila euchromatin.
我们提出了一种基于酵母人工染色体（YAC）构建黑腹果蝇基因组物理图谱的策略。在本文中，我们报告了500个含有平均长度为200 kb的果蝇DNA插入片段的YAC，这些YAC已通过与唾液腺染色体的原位杂交被定位到物理图谱上。克隆的DNA片段具有随机剪切的末端（DY克隆）或由NotI（N克隆）或EcoRI（E克隆）部分消化产生的末端。相对于基因组的常染色质部分，克隆的大小分布和基因组位置显示在基因组覆盖的完整性或随机性方面没有明显偏差。500个定位的常染色质克隆包含总计约1亿个碱基对的DNA，这大约相当于果蝇常染色质的一个基因组当量。
