• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

A new approach using multiplex long accurate PCR and yeast artificial chromosomes for bacterial chromosome mapping and sequencing.

作者信息

Sorokin A, Lapidus A, Capuano V, Galleron N, Pujic P, Ehrlich S D

机构信息

Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France.

出版信息

Genome Res. 1996 May;6(5):448-53. doi: 10.1101/gr.6.5.448.

DOI:10.1101/gr.6.5.448
PMID:8743994
Abstract

An efficient approach for structural studies on bacterial chromosomes is presented. It is based on high-resolution PCR map construction by using a multiplex long accurate PCR (MLA PCR) protocol and a YAC clone carrying the region to be mapped as indicator. The high-resolution PCR map of the bacillus subtilis rrnB-dnaB region is presented as an example. Data are also presented on the use of DNA generated by LA PCR for sequencing; they are relevant to LA PCR induced mutations and justify the application of such mapping for sequencing long stretches of bacterial chromosomes.

摘要

相似文献

1
A new approach using multiplex long accurate PCR and yeast artificial chromosomes for bacterial chromosome mapping and sequencing.
Genome Res. 1996 May;6(5):448-53. doi: 10.1101/gr.6.5.448.
2
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.枯草芽孢杆菌200 kb rrnB - dnaB区域基因的测序与功能注释
Microbiology (Reading). 1997 Nov;143 ( Pt 11):3431-3441. doi: 10.1099/00221287-143-11-3431.
3
Mapping of the 150 kb spoIIIC-pheA region of the Bacillus subtilis chromosome using Long Accurate PCR and three yeast artificial chromosomes.
Microbiology (Reading). 1996 Nov;142 ( Pt 11):3017-20. doi: 10.1099/13500872-142-11-3017.
4
Organization of the Bacillus subtilis 168 chromosome between kdg and the attachment site of the SP beta prophage: use of Long Accurate PCR and yeast artificial chromosomes for sequencing.枯草芽孢杆菌168染色体在kdg与SPβ原噬菌体附着位点之间的组织：利用长片段精确PCR和酵母人工染色体进行测序
Microbiology (Reading). 1996 Nov;142 ( Pt 11):3005-15. doi: 10.1099/13500872-142-11-3005.
5
Sequence analysis of the Bacillus subtilis chromosome region between the serA and kdg loci cloned in a yeast artificial chromosome.对克隆于酵母人工染色体中的枯草芽孢杆菌染色体上serA和kdg基因座之间区域的序列分析。
Microbiology (Reading). 1996 Aug;142 ( Pt 8):2005-16. doi: 10.1099/13500872-142-8-2005.
6
The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism.枯草芽孢杆菌染色体的dnaB-pheA（256°-240°）区域，包含负责应激反应、植物细胞壁利用和初级代谢的基因。
Microbiology (Reading). 1996 Nov;142 ( Pt 11):3067-78. doi: 10.1099/13500872-142-11-3067.
7
Physical mapping of stable RNA genes in Bacillus subtilis using polymerase chain reaction amplification from a yeast artificial chromosome library.利用酵母人工染色体文库中的聚合酶链反应扩增对枯草芽孢杆菌中稳定RNA基因进行物理图谱绘制。
J Bacteriol. 1993 Jul;175(14):4290-7. doi: 10.1128/jb.175.14.4290-4297.1993.
8
The Bacillus subtilis dnaI gene is part of the dnaB operon.枯草芽孢杆菌dnaI基因是dnaB操纵子的一部分。
Microbiology (Reading). 1995 May;141 ( Pt 5):1199-1200. doi: 10.1099/13500872-141-5-1199.
9
Construction of the Bacillus subtilis chromosome physical map and the strategy for mapping newly isolated genes in one membrane filter for hybridization.枯草芽孢杆菌染色体物理图谱的构建以及在一张用于杂交的膜滤器上对新分离基因进行定位的策略。
Nucleic Acids Symp Ser. 1993(29):145-6.
10
"Junction trapping". A simple PCR-based method for the isolation of YAC-insert termini.
Genet Anal Tech Appl. 1993 Apr;10(2):42-8. doi: 10.1016/1050-3862(93)90044-j.

引用本文的文献

1
De Novo Whole-Genome Sequence of Micromonospora carbonacea JXNU-1 with Broad-Spectrum Antimicrobial Activity, Isolated from Soil Samples.从土壤样品中分离得到的具有广谱抗菌活性的碳孢小单孢菌JXNU-1的全基因组序列从头测序
Genome Announc. 2015 Apr 9;3(2):e00174-15. doi: 10.1128/genomeA.00174-15.
2
Genome Sequence of Lactobacillus delbrueckii subsp. lactis CNRZ327, a Dairy Bacterium with Anti-Inflammatory Properties.德氏乳杆菌保加利亚亚种CNRZ327的基因组序列，一种具有抗炎特性的乳杆菌属细菌
Genome Announc. 2014 Jul 17;2(4):e00328-14. doi: 10.1128/genomeA.00328-14.
3
Complete genome sequence of the highly hemolytic strain Bacillus cereus F837/76.
高度溶血菌株蜡样芽胞杆菌 F837/76 的全基因组序列。
J Bacteriol. 2012 Mar;194(6):1630. doi: 10.1128/JB.06719-11.
4
Complete genome sequence of Lactococcus lactis subsp. cremoris A76.乳球菌乳亚种 A76 的全基因组序列。
J Bacteriol. 2012 Mar;194(5):1241-2. doi: 10.1128/JB.06629-11.
5
Complete genome sequence of the clinical Streptococcus salivarius strain CCHSS3.临床唾液链球菌 CCHSS3 株的全基因组序列。
J Bacteriol. 2011 Sep;193(18):5041-2. doi: 10.1128/JB.05416-11. Epub 2011 Jul 8.
6
Complete genome sequence of the commensal Streptococcus salivarius strain JIM8777.共生唾液链球菌 JIM8777 株的全基因组序列。
J Bacteriol. 2011 Sep;193(18):5024-5. doi: 10.1128/JB.05390-11. Epub 2011 Jul 8.
7
The complete genome sequence of Lactobacillus bulgaricus reveals extensive and ongoing reductive evolution.保加利亚乳杆菌的全基因组序列揭示了广泛且持续的还原性进化。
Proc Natl Acad Sci U S A. 2006 Jun 13;103(24):9274-9. doi: 10.1073/pnas.0603024103. Epub 2006 Jun 5.
8
The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403.乳酸乳球菌乳酸亚种IL1403的全基因组序列。
Genome Res. 2001 May;11(5):731-53. doi: 10.1101/gr.gr-1697r.
9
Search and discovery strategies for biotechnology: the paradigm shift.生物技术的搜索与发现策略：范式转变
Microbiol Mol Biol Rev. 2000 Sep;64(3):573-606. doi: 10.1128/MMBR.64.3.573-606.2000.
10
Identification of the lactococcal exonuclease/recombinase and its modulation by the putative Chi sequence.乳酸乳球菌核酸外切酶/重组酶的鉴定及其假定的Chi序列对其的调控
Proc Natl Acad Sci U S A. 1998 Jan 20;95(2):626-31. doi: 10.1073/pnas.95.2.626.