Lapidus Alia, Galleron Nathalie, Sorokin Alexei, Ehrlich S Dusko
Laboratoire de Genetique Microbienne, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy-en-Josas cedex, France.
Microbiology (Reading). 1997 Nov;143 ( Pt 11):3431-3441. doi: 10.1099/00221287-143-11-3431.
The 200 kb region of the Bacillus subtilis chromosome spanning from 255 to 275 degrees on the genetic map was sequenced. The strategy applied, based on use of yeast artificial chromosomes and multiplex Long Accurate PCR, proved to be very efficient for sequencing a large bacterial chromosome area. A total of 193 genes of this part of the chromosome was classified by level of knowledge and biological category of their functions. Five levels of gene function understanding are defined. These are: (i) experimental evidence is available of gene product or biological function; (ii) strong homology exists for the putative gene product with proteins from other organisms; (iii) some indication of the function can be derived from homologies with known proteins; (iv) the gene product can be clustered with hypothetical proteins; (v) no indication on the gene function exists. The percentage of detected genes in each category was: 20, 28, 20, 15 and 17, respectively. In the sequenced region, a high percentage of genes are implicated in transport and metabolic linking of glycolysis and the citric acid cycle. A functional connection of several genes from this region and the genes close to 140 degrees in the chromosome was also observed.
对枯草芽孢杆菌染色体上遗传图谱中跨度为255至275度的200 kb区域进行了测序。所采用的策略基于酵母人工染色体和多重长精确PCR的使用，事实证明该策略对于对大片段细菌染色体区域进行测序非常有效。该染色体这一部分的总共193个基因根据其功能的知识水平和生物学类别进行了分类。定义了五个基因功能理解水平。它们分别是：（i）有基因产物或生物学功能的实验证据；（ii）推定的基因产物与其他生物体的蛋白质存在高度同源性；（iii）可以从与已知蛋白质的同源性中得出功能的一些迹象；（iv）基因产物可以与假设的蛋白质聚类；（v）不存在关于基因功能的迹象。每个类别中检测到的基因百分比分别为：20%、28%、20%、15%和17%。在测序区域中，高比例的基因与糖酵解和柠檬酸循环的转运及代谢连接有关。还观察到该区域的几个基因与染色体上接近140度的基因之间存在功能联系。
