Pappas P W, Wardrop S M
Department of Zoology, Ohio State University, Columbus 43210-1293, USA.
Prep Biochem Biotechnol. 1996 Feb;26(1):53-66. doi: 10.1080/10826069608000050.
Benzoquinones were extracted from flour beetles by rinsing them for 8 min in methanol followed by homogenization in fresh methanol. This procedure recovered > 96% of the total benzoquinones from both species, with the methanolic rinse and supernatant representing the "surface" and "total" benzoquinones. As determined by UV/VIS spectrometry and reversed-phase HPLC, the methanolic rinse contained methyl- and ethyl-1,4-benzoquinone (MBQ and EBQ), but no substituted hydroquinones, The methanolic supernatant contained MBQ and EBQ, and methyl- and ethyl-hydroquinone. When stored at -20 degrees C for 72 hr, the chemical composition of the methanolic rinse did not change, but the composition of the methanolic homogenate did change. Notably, the amounts of benzoquinones in the methanolic supernatant decreased during storage. The method described represents an improvement over those described previously in that it uses less hazardous solvents for extraction (methanol vs more non-polar solvents such as hexane) and allows for the differentiation and quantification of the surface and total benzoquinones of these beetles.
通过将面粉甲虫在甲醇中冲洗8分钟，然后在新鲜甲醇中匀浆，从面粉甲虫中提取苯醌。该程序从两个物种中回收了超过96%的总苯醌，甲醇冲洗液和上清液分别代表“表面”和“总”苯醌。通过紫外/可见光谱法和反相高效液相色谱法测定，甲醇冲洗液中含有甲基-和乙基-1,4-苯醌（MBQ和EBQ），但没有取代对苯二酚，甲醇上清液中含有MBQ、EBQ以及甲基-和乙基-对苯二酚。当在-20℃下储存72小时时，甲醇冲洗液的化学成分没有变化，但甲醇匀浆的成分发生了变化。值得注意的是，甲醇上清液中的苯醌含量在储存期间有所下降。所描述的方法相对于先前描述的方法有所改进，因为它使用危害较小的溶剂进行提取（甲醇与己烷等更多非极性溶剂相比），并且能够区分和定量这些甲虫的表面和总苯醌。
