Corbellino M, Poirel L, Bestetti G, Pizzuto M, Aubin J T, Capra M, Bifulco C, Berti E, Agut H, Rizzardini G, Galli M, Parravicini C
Clinic of Infectious Diseases, University of Milano, L. Sacco Hospital, Italy.
AIDS Res Hum Retroviruses. 1996 May 20;12(8):651-7. doi: 10.1089/aid.1996.12.651.
Specific herpesvirus-like DNA sequences have been found in Kaposi's sarcoma (KS) lesions of AIDS patients, suggesting that a novel gamma herpesvirus, homologous to Epstein-Barr virus and herpesvirus saimiri, could be implicated in the pathogenesis of KS. To better understand the role of this putative etiological agent, named Kaposi's sarcoma-associated herpesvirus (KSHV) or human herpesvirus 8 (HHV-8), we investigated by the polymerase chain reaction (PCR) the presence of viral DNA sequences in various organs obtained at autopsy from seven AIDS patients with KS and six without KS. For each sample, to exclude positive results due to visceral KS dissemination, the presence of microscopic foci of KS cells was rules out by histology and CD34 immunohistochemistry on serial frozen sections immediately adjacent to those employed for DNA extraction. PCR and nested PCR were performed with primers specific for the HIV-8 330 Bam fragment originally described by Chang et al. (Science 1994;266:1865-1869). As quality control, the extracted DNA was amplified with primers for human beta-globin. All KS legions were HHV-8 positive. In addition, extralesional KSHV DNA sequences were detected in seven of seven lymphoid organs and in five of five prostate glands of KS patients. Normal skin was positive in three of five cases and bone marrow in two of three tested cases, all other tissues being negative by PCR and nested PCR. By contrast, no virus was detected in tissue samples of AIDS cases without KS. The restricted organ distribution here documented argues for a selective tissue tropism of HHV-8 in vivo in AIDS patients and suggests that in the infected host lymphoid organs and the prostate gland may represent privileged sites of viral latency and persistence.
在艾滋病患者的卡波西肉瘤（KS）损害中发现了特定的类疱疹病毒DNA序列，这表明一种与爱泼斯坦 - 巴尔病毒和猴疱疹病毒同源的新型γ疱疹病毒可能与KS的发病机制有关。为了更好地理解这种假定的病原体，即卡波西肉瘤相关疱疹病毒（KSHV）或人类疱疹病毒8型（HHV - 8）的作用，我们通过聚合酶链反应（PCR）研究了7例患KS的艾滋病患者和6例未患KS的艾滋病患者尸检时获得的各种器官中病毒DNA序列的存在情况。对于每个样本，为了排除由于内脏KS播散导致的阳性结果，通过组织学和对紧邻用于DNA提取的连续冰冻切片进行CD34免疫组织化学检查，排除KS细胞的微小病灶。使用最初由Chang等人描述的针对HIV - 8 330 Bam片段的引物进行PCR和巢式PCR（《科学》1994年；266:1865 - 1869）。作为质量控制，用人类β - 珠蛋白引物扩增提取的DNA。所有KS病灶均为HHV - 8阳性。此外，在KS患者的7个淋巴器官中的7个以及5个前列腺中的5个中检测到了病灶外的KSHV DNA序列。5例正常皮肤中有3例呈阳性，3例检测的骨髓中有2例呈阳性，通过PCR和巢式PCR检测，所有其他组织均为阴性。相比之下，在未患KS的艾滋病病例的组织样本中未检测到病毒。此处记录的有限器官分布表明HHV - 8在艾滋病患者体内具有选择性组织嗜性，并提示在受感染宿主中，淋巴器官和前列腺可能是病毒潜伏和持续存在的特权部位。
