McDermott C M, Feola R, Plude J
Department of Biology and Microbiology, University of Wisconsin, Oshkosh 54901, USA.
Toxicon. 1995 Nov;33(11):1433-42. doi: 10.1016/0041-0101(95)00095-4.
The development of reliable, sensitive immunoassay techniques for detection of microcystins in water is becoming increasingly important. We have developed an enzyme-linked immunosorbent assay (ELISA) potentially able to detect microcystins at concentrations as low as 95 pg microcystin/ml water. The procedure uses antibodies extracted from the eggs of immunized chickens, eliminating the need to collect blood from laboratory rabbits. The antibody is able to recognize microcystin-LR, and -RR, and may recognize other forms of microcystin. The newly developed ELISA technique was utilized to measure the amount of microcystin in waters of northeastern Wisconsin. Of the water samples analyzed, 87% contained measurable amounts of microcystin (0.2-200 ng/ml). Organisms of the genus Microcystis were identified most frequently from microcystin-containing waters. The distribution of microcystin-producing cyanobacterial strains was apparently random throughout the sampling area.
开发可靠、灵敏的免疫分析技术用于检测水中微囊藻毒素变得越来越重要。我们已开发出一种酶联免疫吸附测定法（ELISA），它有可能检测出低至95皮克微囊藻毒素/毫升水浓度的微囊藻毒素。该程序使用从免疫鸡的卵中提取的抗体，无需从实验兔身上采血。该抗体能够识别微囊藻毒素-LR和-RR，并且可能识别其他形式的微囊藻毒素。新开发的ELISA技术被用于测量威斯康星州东北部水域中的微囊藻毒素含量。在所分析的水样中，87%含有可测量的微囊藻毒素（0.2 - 200纳克/毫升）。在含有微囊藻毒素的水域中，微囊藻属生物最常被鉴定出来。产生微囊藻毒素的蓝藻菌株在整个采样区域的分布显然是随机的。
