Gholami Naser, Mosayebi Mahdi, Dehghan Rahim Abadi Peyman, Rasmi Atigh Hamed, Sedaghat Reza, Naji Zadeh Mohammad Hassan, Farahani Majid
Department of Clinical Science, Faculty of Veterinary Medicine, University of Shahid Chamran, Ahvaz, Iran.
2Department of Parasitology, School of Medicine, Arak University of Medical Sciences, Arāk, Iran.
J Parasit Dis. 2020 Mar;44(1):25-30. doi: 10.1007/s12639-019-01157-9. Epub 2019 Sep 5.
The cestode is a zoonotic tapeworm that it's larval stage which known as cause cyst formation in cattle's organs such as heart, lung, liver, tongue, esophagus and diaphragm muscle, despite the infected cattle may show no clinical signs. Antemortem diagnosis of bovine cysticercosis can be made by antigen detecting ELISA. In a feedlot near city of Arak, beef cattle had different degrees of lethargy, dullness, unthriftiness and were reluctant to move. In postmortem examination of cattle, samples were collected from heart tissue and stained by H&E method for light microscopic examination. 10 ml of blood samples were taken from jugular veins of 90 cattle that were going to be sent to slaughterhouse. Serums obtained from blood samples were investigated for presence of antigen by ELISA assay. Soils and dusts from farm yard, pen's floor, feed store and both toilets of workers and employer were sampled and evaluated for presence of parasite eggs by floating method. antigen were identified in serums of 18 cattle; and also, samples from workers toilet was contaminated by eggs of . This study showed that serologic methods in conjunction with meat inspection can be used for diagnosis of bovine cysticercosis. The aim of this study is to identify infected cattle with by serologic methods before slaughter and determine microscopic characteristics of lesions on postmortem examination in central area of Iran.
这种绦虫是一种人畜共患的绦虫，其幼虫阶段会在牛的心脏、肺、肝脏、舌头、食道和膈肌等器官中形成囊肿，尽管受感染的牛可能没有临床症状。牛囊尾蚴病的生前诊断可通过抗原检测ELISA进行。在阿拉克市附近的一个饲养场，肉牛出现了不同程度的嗜睡、迟钝、消瘦，且不愿走动。在牛的尸体剖检中，从心脏组织采集样本，并用苏木精-伊红（H&E）染色法进行光镜检查。从90头即将被送往屠宰场的牛的颈静脉采集10毫升血样。通过ELISA检测法对血样获得的血清进行抗原检测。对农场院子、牛栏地面、饲料储存处以及工人和雇主的两个厕所的土壤和灰尘进行采样，并通过漂浮法评估寄生虫卵的存在情况。在18头牛的血清中检测到了抗原；此外，工人厕所的样本被该寄生虫的卵污染。本研究表明，血清学方法结合肉类检查可用于诊断牛囊尾蚴病。本研究的目的是在屠宰前通过血清学方法识别感染该寄生虫的牛，并确定伊朗中部地区死后剖检病变的微观特征。
