大鼠单侧肾切除术后可转移的循环因子与上皮钠转运

Transferable circulating factors and epithelial sodium transport after unilateral nephrectomy in the rat.

作者信息

Pollock C A, Nobes M S, Gyory A Z, Heng P T, Field M J

机构信息

Department of Medicine, University of Sydney, NSW, Australia.

出版信息

J Physiol. 1996 Jan 1;490 ( Pt 1)(Pt 1):257-64. doi: 10.1113/jphysiol.1996.sp021141.

DOI:10.1113/jphysiol.1996.sp021141

PMID:8745293

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1158662/

Abstract

In order to investigate the role of circulating serum factors in the altered renal haemodynamics and enhanced renal tubular transport observed in renal growth, micropuncture experiments were performed on normal animals infused with 20% plasma derived from animals in whom unilateral nephrectomy had been performed 3 days previously. 2. When animals infused with plasma from uninephrectomized animals (NxP) were compared with those infused with control plasma, the former had a higher tubular fluid flow rate measured at both the late proximal (LP; 26.7 +/- 1.6 vs. 18.4 +/- 1.4 nl min-1; P < 0.001) and early distal (ED; 14.9 +/- 1.0 vs. 7.8 +/- 1.0 nl min-1; P < 0.0001) sites, which was reflected in the final urine flow rate (16.1 +/- 3.4 vs. 5.1 +/- 0.8 microliter min-1; P < 0.005). 3. The single nephron glomerular filtration rate (SNGFR) was higher in animals infused with NxP as determined from samples taken at the LP (45.8 +/- 2.8 vs. 35.7 +/- 2.3 nl min-1; P < 0.01) and at the ED (34.5 +/- 2.5 vs. 28.1 +/- 1.8 nl min-1; P = 0.05) sites. However, this increase was not reflected in the whole kidney GFR (1.04 +/- 0.06 vs. 0.89 +/- 0.06; P = 0.07), suggestive of a preferential increase in filtration in the outer cortical nephrons. 4. Tubular Na+ transport was higher in the animals infused with NxP as evidenced by a decrease in the fractional delivery of Na+ at the ED site (4.5 +/- 0.4 vs. 6.5 +/- 0.6% of the filtered load; P < 0.05). However, in the final urine there was a significant increase in the urinary sodium excretion in animals infused with NxP (0.67 +/- 0.14 vs. 0.29 +/- 0.09%; P < 0.05) indicating that natriuresis and probably diuresis was a result of inhibition of Na+ and water transport in the late distal tubule and collecting duct. 5. In conclusion, these experiments demonstrate that circulating factors induced by a reduction in renal mass significantly alter glomerular filtration and tubular Na+ transport.

摘要

为了研究循环血清因子在肾脏生长过程中观察到的肾脏血流动力学改变和肾小管转运增强中的作用，对正常动物进行了微穿刺实验，这些动物输注了来自3天前进行单侧肾切除术的动物的20%血浆。2. 将输注来自单侧肾切除动物血浆（NxP）的动物与输注对照血浆的动物进行比较，前者在近端小管晚期（LP；26.7±1.6对18.4±1.4 nl min-1；P<0.001）和远端小管早期（ED；14.9±1.0对7.8±1.0 nl min-1；P<0.0001）部位测得的肾小管液流速更高，这反映在最终尿流率上（16.1±3.4对5.1±0.8微升min-1；P<0.005）。3. 从LP（45.8±2.8对35.7±2.3 nl min-1；P<0.01）和ED（34.5±2.5对28.1±1.8 nl min-1；P = 0.05）部位采集的样本确定，输注NxP的动物单肾单位肾小球滤过率（SNGFR）更高。然而，这种增加并未反映在全肾GFR中（1.04±0.06对0.89±0.06；P = 0.07），提示外皮质肾单位的滤过优先增加。4. 输注NxP的动物肾小管Na+转运更高，这在ED部位Na+的分数排泄减少中得到证明（4.5±0.4对滤过负荷的6.5±0.6%；P<0.05）。然而，在最终尿液中，输注NxP的动物尿钠排泄显著增加（0.67±0.14对0.29±0.09%；P<0.05），表明利钠作用以及可能的利尿作用是远端小管晚期和集合管中Na+和水转运受抑制的结果。5. 总之，这些实验表明肾质量减少诱导的循环因子显著改变肾小球滤过和肾小管Na+转运。