Sundsmo J S, Müller-Eberhard H J
J Immunol. 1979 Jun;122(6):2371-8.
Neoantigenic determinants (neoAg) specific for the assembling membrane attack complex (MAC) of complement were detected by immunofluorescence microscopy on the surface of cytotoxic lymphocytes during the antibody-dependent cellular cytotoxicity (ADCC) reaction. This study employed antibody-sensitized chicken erythrocytes as target cells, human peripheral blood lymphocytes as effector cells, and RITC-conjugated rabbit F(ab')2-anti-neoAg. NeoAg was present on 60% of ADCC plaque-forming lymphocytes (PFL). Eight out of 182 neoAg-positive PFL were observed in direct contact with their target cells. In these cases MAC-specific neoAg was visualized at the zone of contact between the cells. Anti-neoAg Ig was found to inhibit ADCC plaque assays up to 62%; and 51Cr-release assays up to 79%. Stimulation of lymphocytes by PHA or mixed lymphocyte culture increased the expression of neoAg. In the case of PHA, increased neoAg expression was correlated with an increased incorporation of 14C-leucine into C5, C6, C7, and C8 antigens, which was detected by immunodiffusion and autoradiography.
在抗体依赖性细胞毒性（ADCC）反应期间，通过免疫荧光显微镜在细胞毒性淋巴细胞表面检测到针对补体组装膜攻击复合物（MAC）的新抗原决定簇（neoAg）。本研究采用抗体致敏的鸡红细胞作为靶细胞，人外周血淋巴细胞作为效应细胞，以及异硫氰酸罗丹明（RITC）偶联的兔F(ab')2抗新抗原。新抗原存在于60%的ADCC斑块形成淋巴细胞（PFL）上。在182个新抗原阳性的PFL中，有8个观察到与靶细胞直接接触。在这些情况下，MAC特异性新抗原在细胞间接触区域可见。发现抗新抗原Ig可将ADCC斑块试验抑制高达62%；将51Cr释放试验抑制高达79%。用PHA或混合淋巴细胞培养刺激淋巴细胞会增加新抗原的表达。就PHA而言，新抗原表达的增加与14C-亮氨酸掺入C5、C6、C7和C8抗原的增加相关，这通过免疫扩散和放射自显影检测到。
