Sundsmo J S, Curd J G, Kolb W P, Müller-Eberhard H J
J Immunol. 1978 Mar;120(3):855-60.
The specific neoantigenic determinants (neoAg) that are indicative of the assembled C5b-9 C complex are generated on the surface of peripheral blood leukocytes (PBL) during collection and processing of blood. Formation of neoAg on PBL could be prevented by collecting blood directly into 20 mM EDTA and, could be induced in vitro by adding autologous serum to isolated PBL that lacked neoAg. When neoAg was induced by the addition of serum containing 125I-labeled C8, the C8 was incorporated into a 23S complex which could be eluted from PBL. A mechanism for neoAg formation on PBL independent of exogenous serum factors was detected when PBL were placed into culture in serum-free medium. Results with metabolic inhibitors and 14C-leucine suggest that PBL can synthesize C5 and assemble the C5b-9 complex. The possible relevance of these findings to the understanding of mechanisms of cell-mediated cytotoxicity is discussed.
指示组装好的C5b-9 C复合物的特定新抗原决定簇（neoAg）在血液采集和处理过程中在外周血白细胞（PBL）表面产生。通过将血液直接采集到20 mM EDTA中可防止PBL上新抗原的形成，并且通过向缺乏新抗原的分离PBL中添加自体血清可在体外诱导新抗原的形成。当通过添加含125I标记的C8的血清诱导新抗原时，C8被整合到一个可从PBL洗脱的23S复合物中。当将PBL置于无血清培养基中培养时，检测到一种独立于外源性血清因子的PBL上新抗原形成机制。代谢抑制剂和14C-亮氨酸的结果表明，PBL可以合成C5并组装C5b-9复合物。讨论了这些发现与理解细胞介导的细胞毒性机制的可能相关性。
