Hughes B R, Morris C, Cunliffe W J, Leigh I M
Department of Dermatology, London Hospital Medical College, UK.
Br J Dermatol. 1996 Feb;134(2):247-56.
This study compared the pattern of keratin expression in pilosebaceous follicles in uninvolved trunk skin of acne patients, comedones, and normal control skin by immunohistochemistry, using both immunofluorescent (IF) and immunoperoxidase (IP) techniques. The shape, size and gross morphology of truncal follicles varied greatly. There was no difference in keratin expression between normal skin and uninvolved skin of patients with acne. The upper part of the pilosebaceous duct expressed keratins K1, K5, K10 and K14, whereas the lower duct expressed keratins K5, K6, K14, K16, K17 and K19. The sebaceous gland showed considerable heterogeneity in keratin expression, with some lobules expressing keratins K1, K5, K7, K10, K14 and K17. The comedone wall showed a pattern of keratin expression similar to that of the upper follicle, except that there was, in addition, expression of keratins K6 and K16 suprabasally, and panepithelial expression of K17 in the comedone wall. IF techniques were found to be less sensitive than the IP method in the detection of individual keratins, but the use of multiple antibodies and many different transverse and longitudinal sections of follicles permitted full conclusions to be made. The increased expression of keratins K6, K16 and K17 in the comedone wall is likely to represent a secondary effect of increased cell turnover due to the primary underlying mechanism of comedogenesis, which is as yet unknown.
本研究采用免疫荧光（IF）和免疫过氧化物酶（IP）技术，通过免疫组织化学方法比较了痤疮患者未受累躯干皮肤、粉刺及正常对照皮肤中毛囊皮脂腺单位角蛋白的表达模式。躯干毛囊的形状、大小和大体形态差异很大。痤疮患者正常皮肤与未受累皮肤之间的角蛋白表达无差异。毛囊皮脂腺导管上部表达角蛋白K1、K5、K10和K14，而导管下部表达角蛋白K5、K6、K14、K16、K17和K19。皮脂腺在角蛋白表达上表现出相当大的异质性，一些小叶表达角蛋白K1、K5、K7、K10、K14和K17。粉刺壁的角蛋白表达模式与毛囊上部相似，只是在基底层以上还表达角蛋白K6和K16，且粉刺壁全层表达K17。在检测单个角蛋白时，发现IF技术不如IP方法敏感，但使用多种抗体以及对毛囊进行许多不同的横切和纵切切片后，可以得出完整的结论。粉刺壁中角蛋白K6、K16和K17表达增加可能代表由于尚未明确的粉刺形成主要潜在机制导致的细胞更新增加的继发效应。
