Jentzsch A M, Bachmann H, Fürst P, Biesalski H K
Department of Biological Chemistry and Nutrition, University of Hohenheim, Stuttgart, Germany.
Free Radic Biol Med. 1996;20(2):251-6. doi: 10.1016/0891-5849(95)02043-8.
The widely used TBA assay for lipid peroxidation was modified to minimize artefactual oxidative degradation of lipids during the assay. Formation of the TBA-MDA condensation product was studied with and without exclusion of oxygen, and the concentration effect of BHT addition was examined. Oxygen was depleted from the reaction mixture by extensive argon gassing. Exclusion of oxygen resulted in decreased TBARS production in plasma but not in standard solutions. High BHT concentrations resulted in a similar effect. At concentrations higher than 3 mmol/l BHT exclusion of oxygen had no additional effect. By measuring n-butanol extracts in a multititer plate reader this modified method was made suitable as a preliminary screening assay of human body fluids for lipid peroxidation.
对广泛用于检测脂质过氧化的TBA检测法进行了改进，以尽量减少检测过程中脂质的人为氧化降解。在有氧和无氧条件下研究了TBA-MDA缩合产物的形成，并检测了添加BHT的浓度效应。通过大量通入氩气使反应混合物中的氧气耗尽。排除氧气导致血浆中TBARS的生成减少，但标准溶液中未出现这种情况。高浓度的BHT也产生了类似的效果。当BHT浓度高于3 mmol/l时，排除氧气没有额外的效果。通过在多管吸光光度计中测量正丁醇提取物，这种改进后的方法适用于作为人体体液脂质过氧化的初步筛选检测方法。
