Nasir L, Reid S W
Department of Veterinary Medicine, University of Glasgow Veterinary School, Scotland, United Kingdom.
DNA Seq. 1995;6(1):61-3. doi: 10.3109/10425179509074702.
The evolutionary conserved region of the equine homologue of the p53 gene from the donkey genome was PCR amplified and cloned. The 1380 bp fragment consisted of exons 5 to 9 and the intervening introns. The exonic and intronic DNA sequences showed a variable but high level of homology with previously published human sequences. The aminoacid sequences corresponding to the evolutionary conserved domains II, III, and V were identical to the human regions, whilst domain IV was 96% homologous.
从驴基因组中对p53基因的马同源物的进化保守区域进行PCR扩增并克隆。1380 bp的片段由外显子5至9以及其间的内含子组成。外显子和内含子的DNA序列与先前发表的人类序列显示出可变但高度的同源性。对应于进化保守结构域II、III和V的氨基酸序列与人类区域相同，而结构域IV的同源性为96%。
