Nasir L, Reid S W
Department of Veterinary Clinical Studies, Glasgow University Veterinary School, Scotland, United Kingdom.
DNA Seq. 1996;6(3):185-7. doi: 10.3109/10425179609010208.
An evolutionary conserved 1.3 kb fragment corresponding to the horse p53 tumour suppressor gene was PCR amplified, cloned and the nucleotide sequence determined. The p53 fragment encoded exons 5 to 9 and the intervening introns. The nucleotide sequence and the predicted aminoacid sequence showed a high level of homology with human and donkey p53 sequences.
对与马p53肿瘤抑制基因相对应的一段1.3 kb进化保守片段进行聚合酶链反应(PCR)扩增、克隆并测定核苷酸序列。该p53片段编码外显子5至9以及其间的内含子。核苷酸序列和预测的氨基酸序列与人和驴的p53序列显示出高度同源性。