Kraegel S A, Pazzi K A, Madewell B R
Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis 95616, USA.
Cancer Lett. 1995 Jun 8;92(2):181-6. doi: 10.1016/0304-3835(95)03779-v.
An 828 base pair region of canine wild-type p53, corresponding to codons 25 through 312 in the human p53 gene, was directly sequenced from asymmetric polymerase chain reaction (PCR) products. The deduced amino acid sequence of the dog was 86%, 73% and 83% homologous to that of the cat, mouse and human proteins, respectively. In the evolutionarily conserved domains II through V, the canine sequence approached 100% homology with the human sequence. The region sequenced encompasses exons 3-8 where the majority of mutations have been identified in neoplastic specimens from human patients.
从不对称聚合酶链反应（PCR）产物中直接对犬野生型p53的一个828个碱基对的区域进行测序，该区域对应于人p53基因的第25至312密码子。推导的犬氨基酸序列与猫、小鼠和人蛋白质的氨基酸序列分别具有86%、73%和83%的同源性。在进化保守结构域II至V中，犬序列与人序列的同源性接近100%。测序的区域包含外显子3 - 8，在人类患者的肿瘤标本中已鉴定出大部分突变都位于这些外显子中。
