Gilger B C, Andrews J, Wilkie D A, Wyman M, Lairmore M D
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Ohio State University, Columbus 43210-1089, USA.
Vet Immunol Immunopathol. 1995 Dec;49(3):199-208. doi: 10.1016/0165-2427(95)05471-5.
Peripheral cellular immunity of ten dogs with keratoconjunctivitis sicca (KCS) that had not been treated with topical corticosteroids or cyclosporine was evaluated (by use of in vitro lymphocyte proliferation assays and CD4+/CD8+ lymphocyte subset analysis) before and after 1 and 3 months of treatment with topical ocular 2% cyclosporine (CsA). In vitro lymphocyte proliferation and CD4+/CD8+ lymphocyte subset analysis was done in eight normal dogs at the 0, 1 and 3 month time periods to use for comparison. There was no significant difference in lymphocyte proliferation or numbers of CD4+ or CD8+ lymphocytes in dogs with KCS and normal dogs prior to CsA treatment. However, by 1 month's time, lymphocyte proliferation had decreased in the CsA-treated Dogs with KCS, and by 3 months there was a significant difference (P < 0.0001) from the normal dogs. These results suggest that dogs with KCS may not have altered peripheral cellular immunity and that use of topical 2% cyclosporine for treatment of KCS causes a suppression of lymphocyte proliferation after 1 to 3 months of use.
对10只未使用局部皮质类固醇或环孢素治疗的干眼症（KCS）犬的外周细胞免疫进行评估（通过体外淋巴细胞增殖试验和CD4+/CD8+淋巴细胞亚群分析），在局部应用2%环孢素（CsA）治疗1个月和3个月之前及之后进行评估。在0、1和3个月时间段，对8只正常犬进行体外淋巴细胞增殖和CD4+/CD8+淋巴细胞亚群分析以作比较。在CsA治疗前，KCS犬和正常犬的淋巴细胞增殖或CD4+或CD8+淋巴细胞数量无显著差异。然而，到1个月时，CsA治疗的KCS犬的淋巴细胞增殖已降低，到3个月时，与正常犬有显著差异（P < 0.0001）。这些结果表明，KCS犬可能未改变外周细胞免疫，且使用局部2%环孢素治疗KCS在使用1至3个月后会导致淋巴细胞增殖受到抑制。
