Owen A, Bird M
Department of Anatomy, Queen Mary and Westfield College, London, UK.
Neuroreport. 1995 Nov 27;6(17):2269-72. doi: 10.1097/00001756-199511270-00001.
Time-lapse photography was used to examine the effects of acetylcholine on the outgrowth and motility of neurites in cultures of mouse spinal cord. Addition of acetylcholine to the culture medium at concentrations of 100, 10 and 1 microM, caused inhibition of neurite outgrowth. Outgrowth rates were significantly different from those of controls at all concentrations tested. The motility of the growth cones was significantly reduced by treatment with 100 and 10 microM acetylcholine. Blockade of the muscarinic receptor by atropine did not significantly alter the effects of acetylcholine on outgrowth or motility. However when the nicotinic antagonist mecamylamine was added at the same time as acetylcholine the inhibitory effects of the acetylcholine were blocked and no changes in the rate of neurite outgrowth or in growth cone motility were detected. These results suggest that acetylcholine may have a role in the regulation of process outgrowth within the spinal cord with these effects mediated through the nicotinic receptor.
采用延时摄影来研究乙酰胆碱对小鼠脊髓培养物中神经突生长和运动的影响。向培养基中添加浓度为100、10和1微摩尔的乙酰胆碱会抑制神经突生长。在所有测试浓度下，生长速率与对照组均有显著差异。用100和10微摩尔乙酰胆碱处理后，生长锥的运动显著降低。阿托品阻断毒蕈碱受体并没有显著改变乙酰胆碱对生长或运动的影响。然而，当与乙酰胆碱同时添加烟碱拮抗剂美加明时，乙酰胆碱的抑制作用被阻断，未检测到神经突生长速率或生长锥运动的变化。这些结果表明，乙酰胆碱可能在脊髓内突起生长的调节中起作用，且这些作用是通过烟碱受体介导的。
