Ikkai T, Kondo H
Aichi Prefectural University of Fine Arts, Nagakute, Japan.
Biochem Mol Biol Int. 1995 Dec;37(6):1153-61.
Actin in the low ionic strength buffer solution G (0.1 mM ATP, 0.1 mM CaCl2, 2 mM Tris-Cl (pH 8.0), 5 mM 2-mercaptoethanol and 1 mM NaN3) was diluted to the concentration below 10 micrograms/ml at 7 degrees C. Viscometry showed associations of actin monomers and increasing the extent of dilution, maximally about 75% of actin were precipitated. Actin filaments were observed by electron microscope in the diluted solution. Ca2+ plays a role in the filaments' formation. It seems that a phase transition like change in the actin states arose at this concentration range.
肌动蛋白在低离子强度缓冲溶液G（0.1 mM ATP、0.1 mM CaCl2、2 mM Tris-Cl（pH 8.0）、5 mM 2-巯基乙醇和1 mM叠氮化钠）中于7℃下稀释至浓度低于10微克/毫升。粘度测定显示肌动蛋白单体之间存在缔合，并且随着稀释程度增加，最大约75%的肌动蛋白沉淀。在稀释溶液中通过电子显微镜观察到了肌动蛋白丝。Ca2+在丝的形成中起作用。似乎在这个浓度范围内肌动蛋白状态发生了类似相变的变化。
