Inaba D, Duschner H, Jongebloed W, Odelius H, Takagi O, Arends J
Department of Preventive Dentistry, Nagasaki University School of Dentistry, Nagasaki, Japan.
Eur J Oral Sci. 1995 Dec;103(6):368-74. doi: 10.1111/j.1600-0722.1995.tb01859.x.
The effects of a 10% NaOCl treatment for 2 min on demineralized human root dentin were investigated by means of: microradiography (MR), scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM) and secondary ion mass spectroscopy (SIMS). MR measurements revealed that NaOCl caused a tissue contraction not related to water loss but to removal of organic substance(s), resulting in reductions of the lesion depth and mineral loss values by 15% and 42%, respectively. CLSM observations on wet dentin showed that the dentinal tubules underneath the surface are clearly observable and not deformed substantially by the NaOCl, except near the outermost surface. This indicates the importance of wet as well as of dried (high vacuum) observations. SEM micrographs (high vacuum) showed definite changes in the outer dentin surface structure; 85% of the originally open dentinal tubules were closed after NaOCl treatment. No marked changes were observed in the dentin ultrastructure inside lesions, as shown by SEM on fractured surfaces. SIMS data, pertaining to samples in high vacuum, showed a remarkable increase of chlorine (Cl) content in the entire lesion due to the NaOCl, indicating deep penetration of the original OC1 ions. The results suggest that the 2-min treatment of demineralized dentin by NaOCl solutions removes and/or changes part of the dentin matrix in nearly the whole lesion. As a consequence the mineral is somewhat redistributed, the outermost surface of a few mu m is changed, but the main dentin structure and element composition are still intact. These findings indicate that NaOCl treatments are of interest in remineralization and hyper-remineralization studies of dentin.
通过显微放射照相术（MR）、扫描电子显微镜（SEM）、共聚焦激光扫描显微镜（CLSM）和二次离子质谱（SIMS），研究了10%次氯酸钠（NaOCl）处理2分钟对脱矿人牙根牙本质的影响。MR测量显示，NaOCl引起的组织收缩与水分流失无关，而是与有机物质的去除有关，导致病变深度和矿物质流失值分别降低了15%和42%。对湿牙本质的CLSM观察表明，除了最外表面附近，表面下方的牙本质小管清晰可见，且未因NaOCl而发生明显变形。这表明湿态以及干燥（高真空）观察的重要性。SEM显微照片（高真空）显示牙本质外表面结构有明显变化；NaOCl处理后，85%原本开放的牙本质小管被封闭。如断裂表面的SEM所示，病变内部的牙本质超微结构未观察到明显变化。与高真空样品相关的SIMS数据显示，由于NaOCl，整个病变中的氯（Cl）含量显著增加，表明原始OC1离子深度渗透。结果表明，用NaOCl溶液对脱矿牙本质进行2分钟处理可去除和/或改变几乎整个病变中的部分牙本质基质。因此，矿物质有所重新分布，几微米的最外表面发生了变化，但主要牙本质结构和元素组成仍然完整。这些发现表明，NaOCl处理在牙本质再矿化和过度再矿化研究中具有重要意义。
