Inaba D, Ruben J, Takagi O, Arends J
Department of Preventive Dentistry, Nagasaki University School of Dentistry, Nagasaki, Japan.
Caries Res. 1996;30(3):218-24. doi: 10.1159/000262163.
Dentine consists simplified of mineral and of several organic components. Sodium hypochlorite (NaOCl) is a well-known nonspecific proteolytic agent capable to remove organic material. The aim of this study was to investigate the influence of organic material removal from artificial dentine lesions by means of NaOCl pretreatment on subsequent remineralization with and without fluoride. Human root dentine samples were demineralized in an acidic gel (pH = 5) at 37 degrees C for 2 weeks. After 2 min of pretreatment with a 0.4, 2 or 10% NaOCl solution, the samples were remineralized in a 20 mM HEPES buffer (pH = 7) containing 1.5 mM Ca(2) and 0.9 mM phosphate with or without addition of 10 ppm F(-) as NaF at 37 degrees C for 8 days. Mineral profiles were assessed by means of transversal microradiography after diol treatment to avoid shrinkage caused by drying. In a separate experiments the dentine contraction caused by 10% NaOCl was assessed. The contraction (negligible for sound dentine) was found to be about 12% for the lesions. The remineralization results showed that pretreatment with a 10% NaOCl solution for 2 min, increased lesion remineralization. After NaOCl treatment, the amount of accumulated mineral increased by about 27% without F in the remineralization solution, and by about 4% with 10 ppm in solution. The in vitro results suggest that removal of organic materials from dentine lesions is an interesting approach to enhance remineralization.
牙本质主要由矿物质和几种有机成分组成。次氯酸钠（NaOCl）是一种著名的非特异性蛋白水解剂，能够去除有机物质。本研究的目的是调查通过NaOCl预处理去除人工牙本质病变中的有机物质对随后有无氟再矿化的影响。人牙根牙本质样本在37℃的酸性凝胶（pH = 5）中脱矿2周。在用0.4%、2%或10%的NaOCl溶液预处理2分钟后，样本在含有1.5 mM Ca(2)和0.9 mM磷酸盐的20 mM HEPES缓冲液（pH = 7）中再矿化8天，再矿化溶液中添加或不添加10 ppm F(-)作为NaF。在二醇处理后通过横向显微放射照相术评估矿物质分布，以避免干燥引起的收缩。在一个单独的实验中，评估了10% NaOCl引起的牙本质收缩。发现病变的收缩率（健康牙本质可忽略不计）约为12%。再矿化结果表明，用10%的NaOCl溶液预处理2分钟可增加病变再矿化。NaOCl处理后，再矿化溶液中无氟时积累的矿物质增加约27%，溶液中有10 ppm氟时增加约4%。体外结果表明，从牙本质病变中去除有机物质是增强再矿化的一种有趣方法。
