两种骨髓六肽（骨髓肽）在免疫缺陷实验模型中的免疫调节作用

Immunoregulatory effects of two bone-marrow hexapeptides (myelopeptides) in experimental models of immunodeficiency.

作者信息

Mikhailova A A, Shanurin S Y, Petrov R V

机构信息

Department of Immunology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.

出版信息

Immunol Lett. 1995 Sep;47(3):199-203. doi: 10.1016/0165-2478(95)00086-1.

DOI:10.1016/0165-2478(95)00086-1

PMID:8747719

Abstract

Myelopeptide 1 (MP-1, Phe-Leu-Gly-Phe-Pro-Thr) and myelopeptide 2 (MP-2, Leu-Val-Val-Tyr-Pro-Trp) are hexapeptides originally isolated from porcine bone-marrow cell culture. MP-1 enhanced the number of antibody-forming cells in NZB mouse spleen cells in vitro and increased the antibody production in Cy-treated (CBAxC57B1/6)F1 mice in vivo. MP-1 increased ConA-induced proliferation of both NZB spleen cells in vitro and spleen cells of Cy-treated mice in vivo. MP-1 restored polyclonal IgG synthesis depressed by PWM in the NZB spleen cell culture. MP-2 also evoked immunoregulatory effects which were less pronounced and realized at higher concentrations than effects of MP-1. Neither MP-1 nor MP-2 affected LPS-induced spleen cell proliferation in both NZB- and Cy-treated (CBAxC57B1/6)F1 mice.

摘要

髓肽1（MP-1，苯丙氨酸-亮氨酸-甘氨酸-苯丙氨酸-脯氨酸-苏氨酸）和髓肽2（MP-2，亮氨酸-缬氨酸-缬氨酸-酪氨酸-脯氨酸-色氨酸）是最初从猪骨髓细胞培养物中分离出来的六肽。MP-1在体外增强了NZB小鼠脾细胞中抗体形成细胞的数量，并在体内增加了环磷酰胺处理的（CBAxC57B1/6）F1小鼠的抗体产生。MP-1在体外增加了ConA诱导的NZB脾细胞以及体内环磷酰胺处理小鼠脾细胞的增殖。MP-1恢复了NZB脾细胞培养物中被PWM抑制的多克隆IgG合成。MP-2也具有免疫调节作用，但其作用不如MP-1明显，且在高于MP-1的浓度下才能实现。MP-1和MP-2均不影响NZB和环磷酰胺处理的（CBAxC57B1/6）F1小鼠中LPS诱导的脾细胞增殖。