从猪骨髓细胞培养物中分离出的六肽的免疫调节特性

Immunoregulatory properties of hexapeptide isolated from porcine bone marrow cell culture.

作者信息

Mikhailova A, Fonina L, Kirilina E, Shanurin S, Gur'yanov S, Malakhov A, Nesmeyanov V, Petrov R

机构信息

Department of Immunology, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.

出版信息

Regul Pept. 1994 Oct 21;53(3):203-9. doi: 10.1016/0167-0115(94)90169-4.

DOI:10.1016/0167-0115(94)90169-4

PMID:7846296

Abstract

Myelopeptide 1 (MP-1) is hexapeptide originally isolated from porcine bone marrow cell culture. It was synthesized and its immunoregulatory properties were studied. MP-1 caused a 1.5-2-fold dose-dependent increase of antibody production in the culture of mouse immune lymph node cells. It abolished Con A induction of T suppressors in the suspension of mouse spleen cells and counteracted the inhibitory effect of T suppressors on antibody production. The inoculation of MP-1 (1 x 10(-9) g/mouse) to mice two weeks after their gamma-irradiation (2 Gy) resulted in an increase of antibody production up to 80.2 +/- 15.5% as compared to that in the irradiated control 37.6 +/- 12.0%. Immunofluorescent analysis revealed the specific binding of MP-1 with receptors on the target cells in the suspension of mouse spleen cells. It is supposed that MP-1 participates in the immunoregulatory processes in the living organism.

摘要

肌肽 1（MP - 1）是最初从猪骨髓细胞培养物中分离出的六肽。它被合成并对其免疫调节特性进行了研究。MP - 1 在小鼠免疫淋巴结细胞培养中引起抗体产生呈 1.5 至 2 倍的剂量依赖性增加。它消除了小鼠脾细胞悬液中伴刀豆球蛋白 A 对 T 抑制细胞的诱导作用，并抵消了 T 抑制细胞对抗体产生的抑制作用。在小鼠接受γ射线照射（2 Gy）两周后给其接种 MP - 1（1×10⁻⁹ g/小鼠），与照射对照组（37.6±12.0%）相比，抗体产生增加了 80.2±15.5%。免疫荧光分析揭示了 MP - 1 与小鼠脾细胞悬液中靶细胞上的受体有特异性结合。推测 MP - 1 参与生物体内的免疫调节过程。