McCluskey B J, Greiner E C, Donovan G A
US Department of Agriculture, APHIS, Englewood, CO 80155, USA.
Vet Parasitol. 1995 Dec;60(3-4):185-90. doi: 10.1016/0304-4017(95)00790-4.
Cryptosporidium spp. oocyst shedding was observed in calves from approximately 1 to 30 days of age. Oocysts were detected by either the Kinyoun acid-fast staining technique (microscopic examination--ME) or a commercially produced enzyme immunoassay EIA). Test concordance between the two detection methods was determined. The mean (+/- SD) number of days to detection of cryptosporidial oocysts was 9.52 +/- 1.92 for the ME and 9.83 +/- 3.19 for the EIA. No significant difference between the means was found (P = 0.17). The period prevalence of cryptosporidiosis was 100% in calves from 1 to 30 days of age. The overall agreement between the ME and EIA was 72%, with a kappa value of 0.42 (SE +/- 0.05). McNemar's test indicated that the proportion of tests determined positive by the two methods was not equal (P < 0.01). The findings or this study indicate moderate agreement between the two diagnostic methods, with the EIA being the more sensitive of the two. However, in most cases the herd-level determination of cryptosporidiosis requires minimal sample sizes and is more economically and easily accomplished by the ME method of detection.
在大约1至30日龄的犊牛中观察到隐孢子虫属卵囊排出。通过金胺酚抗酸染色技术（显微镜检查 - ME）或市售酶免疫测定法（EIA）检测卵囊。确定了两种检测方法之间的测试一致性。ME检测隐孢子虫卵囊的平均（±标准差）天数为9.52±1.92天，EIA为9.83±3.19天。均值之间未发现显著差异（P = 0.17）。1至30日龄犊牛隐孢子虫病的期间患病率为100%。ME和EIA之间的总体一致性为72%，kappa值为0.42（标准误±0.05）。McNemar检验表明两种方法判定为阳性的检测比例不相等（P < 0.01）。本研究结果表明两种诊断方法之间具有中等一致性，其中EIA在两者中更敏感。然而，在大多数情况下，群体水平的隐孢子虫病测定所需样本量最少，通过ME检测方法在经济上和操作上更容易实现。
