Vanrompay D, Cox E, Kaiser P, Lawson S, Van Loock M, Volckaert G, Goddeeris B
Ghent University, Department of Molecular Biotechnology, Belgium.
Immunology. 2001 May;103(1):106-12. doi: 10.1046/j.1365-2567.2001.01215.x.
Plasmid DNA (pcDNA1::MOMP A) expressing the major outer membrane protein of an avian Chlamydophila psittaci serovar A strain was tested for its ability to induce protective immunity against challenge with the same C. psittaci serovar. A combined parenteral (intramuscular injection) and mucosal route (DNA drops administered to the nares) of DNA inoculation was compared to three other, different routes of administration (intramuscular inoculation, DNA drops administered to the nares and aerosol immunization). In addition, the effect of turkey interferon gamma (tIFN-gamma) on intramuscular immunization was evaluated by co-expressing pCIneo::tIFN-gamma. A significant level of protection was observed in turkeys immunized via the combined parenteral/mucosal route, the intramuscular route or by aerosol. Severe clinical signs and lesions were observed in the non-vaccinated control groups, in 80% of turkeys inoculated with a mixture of pcDNA1::MOMP A and pCIneo::tIFN-gamma, and in 60% of turkeys vaccinated with DNA drops administered to the nares. The use of MOMP-based DNA vaccination as a means of preventing severe clinical signs and lesions in a turkey model of C. psittaci infection was demonstrated, as was down-regulation of the immune response by co-expression of tIFN-gamma.
对表达禽鹦鹉热衣原体A血清型菌株主要外膜蛋白的质粒DNA（pcDNA1::MOMP A）进行了测试，以评估其诱导针对相同鹦鹉热衣原体血清型攻击的保护性免疫的能力。将DNA接种的联合肠胃外（肌肉注射）和粘膜途径（滴鼻给予DNA滴剂）与其他三种不同的给药途径（肌肉接种、滴鼻给予DNA滴剂和气溶胶免疫）进行了比较。此外，通过共表达pCIneo::tIFN-γ评估了火鸡干扰素γ（tIFN-γ）对肌肉免疫的影响。通过联合肠胃外/粘膜途径、肌肉途径或气溶胶免疫的火鸡观察到了显著水平的保护作用。在未接种疫苗的对照组、80%接种pcDNA1::MOMP A和pCIneo::tIFN-γ混合物的火鸡以及60%滴鼻接种DNA滴剂的火鸡中观察到了严重的临床症状和病变。证明了基于MOMP的DNA疫苗接种作为预防鹦鹉热衣原体感染火鸡模型中严重临床症状和病变的一种手段，同时也证明了通过共表达tIFN-γ对免疫反应的下调作用。
