Chanez P, Vignola M, Stenger R, Vic P, Michel F B, Bousquet J
Clinique des Maladies Respiratoires, Hôpital Arnaud de Villeneuve, Centre Hospitalier Universitaire, Montpellier, France.
Allergy. 1995 Nov;50(11):878-83. doi: 10.1111/j.1398-9995.1995.tb02493.x.
Platelet-derived growth factor (PDGF) controls cellular growth, migration, and differentiation. It is secreted by various cell types, including macrophages, and participates in tissue repair and epithelial regeneration. PDGF may therefore be involved in airway remodeling in asthma. This study compared the immunoreactivity of PDGF and its receptors (R alpha and R beta) in bronchial biopsies and the levels of PDGF in bronchoalveolar lavage (BAL) fluid of asthmatics and control subjects. Bronchial biopsies were done in a subsegmental bronchus of 11 asthmatics and 11 control subjects by flexible bronchoscope. PDGF AA and BB, and PDGF receptors R alpha and R beta were studied with monoclonal antibodies and revealed by immunoperoxidase staining. The percentage of subjects presenting positive staining with PDGFs and its receptors was studied in the epithelium and submucosa. PDGF AA, AB, and BB were measured in BAL fluid of 18 asthmatics and 10 controls by specific ELISA. In biopsies, there was no significant difference between asthmatics and controls for PDGF AA, BB, PDGF-R alpha and R beta (Fisher's exact test and Bonferroni's correction). Moreover, the levels of PDGF, AA, AB, and BB were similar in asthmatics and controls. This study does not support a role for PDGF in the repair processes of asthma.
血小板衍生生长因子（PDGF）控制细胞的生长、迁移和分化。它由包括巨噬细胞在内的多种细胞类型分泌，并参与组织修复和上皮再生。因此，PDGF可能参与哮喘的气道重塑。本研究比较了哮喘患者和对照受试者支气管活检中PDGF及其受体（Rα和Rβ）的免疫反应性以及支气管肺泡灌洗（BAL）液中PDGF的水平。通过柔性支气管镜对11例哮喘患者和11例对照受试者的亚段支气管进行了支气管活检。用单克隆抗体研究了PDGF AA和BB以及PDGF受体Rα和Rβ，并通过免疫过氧化物酶染色显示。研究了上皮和黏膜下层中PDGF及其受体呈阳性染色的受试者百分比。通过特异性ELISA检测了18例哮喘患者和10例对照受试者BAL液中的PDGF AA、AB和BB。在活检中，哮喘患者和对照受试者在PDGF AA、BB、PDGF-Rα和Rβ方面无显著差异（Fisher精确检验和Bonferroni校正）。此外，哮喘患者和对照受试者的PDGF、AA、AB和BB水平相似。本研究不支持PDGF在哮喘修复过程中起作用。
