Sur S, Gleich G J, Offord K P, Swanson M C, Ohnishi T, Martin L B, Wagner J M, Weiler D A, Hunt L W
Department of Medicine, East Carolina University School of Medicine, Greenville, NC 27858-4354, USA.
Allergy. 1995 Nov;50(11):891-8. doi: 10.1111/j.1398-9995.1995.tb02495.x.
To test whether eosinophil recruitment after pulmonary allergen challenge is associated with interleukin (IL)-5 in patients with asthma, we performed segmental bronchoprovocation (SBP) with saline, and with low and high dosages of ragweed extract in six patients with allergic asthma. Bronchoalveolar lavage (BAL) of the challenged segments was performed 5 min after challenge (immediate BAL fluid) and repeated 24 h later (late BAL fluid). Allergen challenge resulted in recruitment of eosinophils, and increased levels of eosinophil-active cytokines. A bioassay showed the predominant eosinophil-active cytokine in the late BAL fluids to be IL-5. Analysis of the late BAL fluids revealed that IL-5 levels correlated with the numbers of eosinophils and lymphocytes. This study provides evidence that IL-5 is a critical cytokine associated with eosinophil and lymphocyte recruitment into the airways of patients with asthma following exposure to allergen.
为了检测哮喘患者肺部过敏原激发后嗜酸性粒细胞募集是否与白细胞介素（IL）-5相关，我们对6例过敏性哮喘患者进行了盐水、低剂量和高剂量豚草提取物的节段性支气管激发试验（SBP）。激发后5分钟（即时支气管肺泡灌洗（BAL）液）对激发节段进行支气管肺泡灌洗，并在24小时后重复（迟发BAL液）。过敏原激发导致嗜酸性粒细胞募集，并使嗜酸性粒细胞活性细胞因子水平升高。生物测定显示，迟发BAL液中主要的嗜酸性粒细胞活性细胞因子是IL-5。对迟发BAL液的分析显示，IL-5水平与嗜酸性粒细胞和淋巴细胞数量相关。本研究提供了证据，表明IL-5是一种关键细胞因子，与哮喘患者暴露于过敏原后嗜酸性粒细胞和淋巴细胞向气道募集有关。
