Ohnishi T, Sur S, Collins D S, Fish J E, Gleich G J, Peters S P
Department of Medicine, Jefferson Medical College, Philadelphia, Pa 19107-5083.
J Allergy Clin Immunol. 1993 Oct;92(4):607-15. doi: 10.1016/0091-6749(93)90085-t.
Segmental antigen challenge in allergic volunteer subjects leads to the recruitment of inflammatory cells, including eosinophils, to the lung and to lung injury as shown by albumin influx into the alveolar air space. The goal of this study was to determine whether eosinophil-active cytokines, including IL-3, IL-5, or granulocyte-macrophage colony-stimulating factor, are released into the lung 24 hours after segmental antigen challenge of ragweed allergic subjects with allergic rhinitis and to determine whether the presence of the cytokine or cytokines is correlated with markers of lung inflammation and lung injury.
Volunteers underwent challenge with a wide variety of antigen doses, which resulted in the recruitment of inflammatory cell mixtures both with and without eosinophils.
Eosinophil survival activity (ESA), the ability of the cytokine to prolong blood eosinophil survival in culture, was found in 5 of 17 ragweed allergic subjects and only in subjects challenged with relatively high doses of ragweed antigen (0.2 ragweed antigen units/ml or more). No ESA was found in bronchoalveolar lavage (BAL) fluid in any of eight nonragweed allergic subjects. This activity could be almost completely neutralized by preincubating BAL fluid with specific antibody to IL-5, although a small contribution by granulocyte-macrophage colony-stimulating factor may also have been present. ESA correlated with eosinophil recruitment (r = 0.72, p < 0.001) and degranulation in the lung (r = 0.63 to 0.81, p < 0.01, for eosinophil granule constituents in BAL fluid) and lung injury as shown by albumin influx into the alveolar air spaces (r = 0.83, p < 0.001). ESA was unrelated to the presence of other inflammatory cells in the lung. Subjects who had IL-5 in BAL fluid appeared to undergo more severe initial reactions to antigen challenge.
We conclude that IL-5 is the most important constituent in ESA in the lung 24 hours after antigen challenge and that it correlates with eosinophil recruitment, degranulation, and lung injury.
在变应性志愿者受试者中进行节段性抗原激发会导致炎症细胞（包括嗜酸性粒细胞）募集至肺部，并导致肺损伤，如白蛋白流入肺泡腔所示。本研究的目的是确定在患有过敏性鼻炎的豚草变应性受试者进行节段性抗原激发24小时后，包括白细胞介素-3（IL-3）、白细胞介素-5（IL-5）或粒细胞-巨噬细胞集落刺激因子在内的嗜酸性粒细胞活性细胞因子是否释放至肺部，以及该细胞因子或多种细胞因子的存在是否与肺部炎症和肺损伤标志物相关。
志愿者接受了多种抗原剂量的激发，这导致了有嗜酸性粒细胞和无嗜酸性粒细胞的炎症细胞混合物的募集。
在17名豚草变应性受试者中的5名中发现了嗜酸性粒细胞存活活性（ESA），即细胞因子在培养中延长血液嗜酸性粒细胞存活的能力，且仅在接受相对高剂量豚草抗原（0.2豚草抗原单位/毫升或更高）激发的受试者中发现。在8名非豚草变应性受试者的支气管肺泡灌洗（BAL）液中均未发现ESA。通过用IL-5特异性抗体预孵育BAL液，该活性几乎可被完全中和，尽管粒细胞-巨噬细胞集落刺激因子可能也有小部分作用。ESA与嗜酸性粒细胞募集（r = 0.72，p < 0.001）、肺内脱颗粒（对于BAL液中的嗜酸性粒细胞颗粒成分，r = 0.63至0.81，p < 0.01）以及白蛋白流入肺泡腔所示的肺损伤（r = 0.83，p < 0.001）相关。ESA与肺内其他炎症细胞的存在无关。BAL液中有IL-5的受试者似乎对抗原激发的初始反应更严重。
我们得出结论，IL-5是抗原激发24小时后肺内ESA的最重要成分，且它与嗜酸性粒细胞募集、脱颗粒和肺损伤相关。
