McBride W J, Bodart B, Lumeng L, Li T K
Department of Psychiatry, Institute of Psychiatric Research, Indiana University; School of Medicine, Indianapolis 46202-4887, USA.
Alcohol Clin Exp Res. 1995 Dec;19(6):1420-2. doi: 10.1111/j.1530-0277.1995.tb01001.x.
The contents of dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), serotonin (5-HT), and 5-hydroxyindoleacetic acid (5-HIAA) were determined in the nucleus accumbens (ACB), frontal cortex (FR), anterior striatum (AST), and hippocampus (HIP) of adult male rats from the F2 generation of P x NP intercrosses. Rats were tested for their alcohol preference and were divided into two groups, depending on their alcohol intake. Rats in the high drinking group (n = 11) had ethanol intakes > 5g/kg/day, whereas the low drinking group (n = 15) had values < 1 g/kg/day. The content of DA in the ACB was lower (p < 0.001) in the high alcohol drinking group (46 +/- 2 pmol/mg tissue) than in the low intake rats (61 +/- 3 pmol/mg tissue). However, the contents of DOPAC and HVA in the ACB were similar for both groups. There were no differences between the two groups in the contents of DA in the FR or AST. The content of 5-HT in the ACB was lower (p < 0.05) in high alcohol drinking rats (6.3 +/- 0.3 pmol/mg tissue) than in the low intake group (7.0 +/- 0.2 pmol/mg tissue). The content of 5-HIAA in the ACB of the high intake rats was also lower than the level for the low drinking rats. There were no differences in the contents of 5-HT or 5-HIAA in the FR, HIP, and AST between the two groups. The results confirm a phenotypic association between abnormal DA and 5-HT systems projecting to the ACB and high alcohol drinking behavior in the P line of rats.
在P与NP杂交F2代成年雄性大鼠的伏隔核（ACB）、额叶皮质（FR）、前纹状体（AST）和海马体（HIP）中测定了多巴胺（DA）、3,4 - 二羟基苯乙酸（DOPAC）、高香草酸（HVA）、5 - 羟色胺（5 - HT）和5 - 羟基吲哚乙酸（5 - HIAA）的含量。根据大鼠的酒精摄入量对其进行测试，并将其分为两组。高饮酒组（n = 11）的乙醇摄入量>5g/kg/天，而低饮酒组（n = 15）的乙醇摄入量<1g/kg/天。高酒精摄入组ACB中DA的含量（46±2 pmol/mg组织）低于低摄入组大鼠（6１±3 pmol/mg组织）（p < 0.001）。然而，两组ACB中DOPAC和HVA的含量相似。两组在FR或AST中DA的含量没有差异。高酒精摄入大鼠ACB中5 - HT的含量（6.3±0.3 pmol/mg组织）低于低摄入组（7.0±0.2 pmol/mg组织）（p < 0.05）。高摄入大鼠ACB中5 - HIAA的含量也低于低饮酒大鼠。两组在FR、HIP和AST中5 - HT或5 - HIAA的含量没有差异。结果证实了投射到ACB的异常DA和5 - HT系统与大鼠P系中高酒精饮用行为之间的表型关联。
