Grobler J, Bauer F, Subden R E, Van Vuuren H J
Department of Microbiology and Institute for Biotechnology, University of Stellenbosch, South Africa.
Yeast. 1995 Dec;11(15):1485-91. doi: 10.1002/yea.320111503.
The mae1 gene of the yeast Schizosaccharomyces pombe was identified on the basis of its ability to complement a mutant defective in the transport of malic acid. Analysis of the DNA sequence revealed an open reading frame of 1314 base pairs, encoding a polypeptide of 438 amino acids with a predicted molecular weight of 49 kDa. A hydropathy profile of the predicted amino acid sequence revealed a protein with ten membrane-spanning or associated domains and hydrophilic N- and C- termini. The predicted secondary structure of the protein in similar to models proposed for other integral membrane proteins from both prokaryotes and eukaryotes. The S. pombe mae1 gene encodes a single mRNA of 1.5 kb. The mea1 gene is expressed constitutively and is not subject to catabolite repression as was previously reported for the malate permease systems of Candida utilis and Hansenula anomala. The mae1 gene was mapped 2842 bp 5' to the MFml gene on chromosome I. Transport assays revealed that the mae1 gene encodes a permease involved in the uptake of L-malate, succinate and malonic acid.
粟酒裂殖酵母的mae1基因是根据其对苹果酸转运缺陷型突变体的互补能力而鉴定出来的。DNA序列分析揭示了一个1314个碱基对的开放阅读框，编码一个438个氨基酸的多肽，预测分子量为49 kDa。预测氨基酸序列的亲水性图谱显示该蛋白质具有十个跨膜或相关结构域以及亲水性的N端和C端。该蛋白质的预测二级结构与针对原核生物和真核生物的其他整合膜蛋白提出的模型相似。粟酒裂殖酵母mae1基因编码一个1.5 kb的单一mRNA。mae1基因组成型表达，并且不像先前报道的产朊假丝酵母和异常汉逊酵母的苹果酸通透酶系统那样受到分解代谢物阻遏。mae1基因定位于染色体I上MFml基因5'端2842 bp处。转运分析表明，mae1基因编码一种参与L-苹果酸、琥珀酸和丙二酸摄取的通透酶。
