Petroff O A, Pleban L A, Spencer D D
Department of Neurology, Yale University, New Haven, CT 06510, USA.
Magn Reson Imaging. 1995;13(8):1197-211. doi: 10.1016/0730-725x(95)02033-p.
High resolution 1H NMR spectroscopy was used to analyze temporal lobe biopsies obtained from patients with epilepsy. Heat-stabilized cerebrum, dialyzed cytosolic macromolecules, and perchloric acid extracts were studied using one- and two-dimensional spectroscopy. Anterior temporal lobe neocortex was enriched in GABA, glutamate, alanine, N-acetylaspartate, and creatine. Subjacent white matter was enriched in aspartate, glutamine, and inositol. The N-acetylaspartate/creatine mole ratio was lower in anterior temporal neocortex with mesial (0.66) than neocortical (0.80) temporal lobe epilepsy. Human brain biopsy samples were separated into crude and refined synaptosomes, neuronal cell bodies, and glia using density gradient centrifugation. Neuronal fractions were enriched in glutamate and N-acetylaspartate. Glial cell fractions were enriched in lactate, glutamine, and inositol. The creatine content was the same in biopsied epileptic cortex (8.8-8.9 mmol/kg) and normal in vivo occipital lobe (8.9 mmol/kg). Glutamate content was higher in epileptic cortex at biopsy (10.1-10.5 mmol/kg) than normal in vivo occipital lobe (8.8 mmol/kg). GABA content was higher in biopsies of epileptic cortex (2.3-2.2 mmol/kg) than in normal in vivo occipital lobe (1.2 mmol/kg). N-acetylaspartate content was lower in biopsied epileptic temporal cortex (5.8-6.8 mmol/kg) than normal in vivo occipital lobe (8.9 mmol/kg). Paired in vivo and ex vivo measurements are critical for a firm understanding of the changes seen in the 1H-spectra from patients with epilepsy.
高分辨率1H核磁共振波谱用于分析癫痫患者颞叶活检样本。使用一维及二维波谱对热稳定化大脑、透析后的胞质大分子和高氯酸提取物进行研究。颞叶前部新皮质富含γ-氨基丁酸、谷氨酸、丙氨酸、N-乙酰天门冬氨酸和肌酸。其下方白质富含天冬氨酸、谷氨酰胺和肌醇。内侧颞叶癫痫患者的颞叶前部新皮质中N-乙酰天门冬氨酸/肌酸摩尔比（0.66）低于新皮质颞叶癫痫患者（0.80）。通过密度梯度离心将人脑活检样本分离为粗制和精制突触体、神经元细胞体及神经胶质。神经元部分富含谷氨酸和N-乙酰天门冬氨酸。神经胶质细胞部分富含乳酸、谷氨酰胺和肌醇。活检的癫痫皮质中肌酸含量（8.8 - 8.9 mmol/kg）与正常枕叶活体组织（8.9 mmol/kg）相同。癫痫皮质活检时谷氨酸含量（10.1 - 10.5 mmol/kg）高于正常枕叶活体组织（8.8 mmol/kg）。癫痫皮质活检样本中γ-氨基丁酸含量（2.3 - 2.2 mmol/kg）高于正常枕叶活体组织（1.2 mmol/kg）。癫痫颞叶皮质活检样本中N-乙酰天门冬氨酸含量（5.8 - 6.8 mmol/kg）低于正常枕叶活体组织（8.9 mmol/kg）。体内和体外配对测量对于深入理解癫痫患者1H谱中所见变化至关重要。
