Willingham A L, Bøgh H O, Vennervald B J, Johansen M V, Eriksen L, Christensen N O, Nansen P
Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Vet Parasitol. 1996 Jan;61(1-2):157-63. doi: 10.1016/0304-4017(95)00810-1.
Worm establishment and egg production were compared in Landrace/Yorkshire crossbred pigs infected with Schistosoma japonicum using 3 different infection techniques. S. japonicum worm establishment and overall egg production following intramuscular injection of cercariae suspended in Iscove's medium exceeded that in pigs infected by the leg immersion or the coverslip percutaneous technique. However, the egg production per mature female was similar for all three infection methods. The results support the use of intramuscular injection of medium-suspended cercariae as an effective, safe, rapid and convenient infection method for studies on the population biology of S. japonicum in pigs when dermal cercarial penetration is not required.
使用3种不同的感染技术，比较了长白/约克夏杂交猪感染日本血吸虫后的虫体建立情况和产卵量。肌肉注射悬浮于伊斯科夫培养基中的尾蚴后，日本血吸虫的虫体建立情况和总产卵量超过了通过腿部浸泡或盖玻片经皮技术感染的猪。然而，三种感染方法中每只成熟雌虫的产卵量相似。结果支持，当不需要尾蚴经皮肤穿透时，肌肉注射悬浮于培养基中的尾蚴可作为一种有效、安全、快速且便捷的感染方法，用于猪体内日本血吸虫种群生物学的研究。
